21 Feb 2023
 | 21 Feb 2023
Status: this preprint is open for discussion.

Increase of bacterial community induced-tolerance to Cr in response to soil properties and Cr level in soil

Claudia Campillo-Cora, Daniel Arenas-Lago, Manuel Arias-Estévez, and David Fernández-Calviño

Abstract. Chromium (Cr) pollution in soils is a global concern that should be assessed. Pollution Induced Community Tolerance (PICT) methodology is a highly sensitive tool that can directly indicate metal toxicity in the microbial community. Ten soils with a wide range of properties were spiked with 31.25, 62.5, 125, 250, 500, 1000 and 2000 mg Cr·kg-1, in addition to the control. Bacterial growth (using [3H]-leucine incorporation technique) was used to determine PICT, that is, whether bacterial communities developed tolerance in response to Cr additions to different soil types. Some bacterial communities did not grow normally at 1000 or 2000 mg Cr·kg-1, probably due to high Cr toxicity, while others did. Regarding below 500 mg Cr·kg-1, bacterial communities showed two responses depending on soil type: 7 of the 10 studied soils showed increased tolerance to Cr, while for the remaining 3 soils did not develop tolerance to Cr. Furthermore, the Cr level from which bacterial communities developed tolerance was dependent on the soil, i.e. Cr was more toxic in some of studied soils. The Cr effect on microbial communities was mainly determined by Dissolved Organic Carbon (DOC) and the fraction of Cr extracted with distilled water (H2O-Cr) (R2 = 95.6 %). Their effect on Cr in the soil might lead to an increase in toxicity (selection phase of PICT).

Claudia Campillo-Cora et al.

Status: open (until 23 Apr 2023)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse

Claudia Campillo-Cora et al.

Claudia Campillo-Cora et al.


Total article views: 123 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
98 20 5 123 17 3 3
  • HTML: 98
  • PDF: 20
  • XML: 5
  • Total: 123
  • Supplement: 17
  • BibTeX: 3
  • EndNote: 3
Views and downloads (calculated since 21 Feb 2023)
Cumulative views and downloads (calculated since 21 Feb 2023)

Viewed (geographical distribution)

Total article views: 116 (including HTML, PDF, and XML) Thereof 116 with geography defined and 0 with unknown origin.
Country # Views %
  • 1
Latest update: 29 Mar 2023
Short summary
Cr pollution is a global concern. The use of methodologies specifically related to Cr toxicity is appropriate: Pollution-Induced Community Tolerance (PICT) methodology. The development of PICT was determined in 10 soils after Cr addition in laboratory. Cr soluble fraction and dissolved organic carbon were the main variables determining the development of PICT (R2 = 95.6 %).