Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water extractable colloids of a Vertisol

Bai, Shanshan; Ge, Yifei; Yao, Dongtan; Wang, Yifan; Tan, Jinfang; Zhang, Shuai; Peng, Yutao; Jiang, Xiaoqian

doi:https://doi.org/10.5194/egusphere-2024-983

Preprints

https://doi.org/10.5194/egusphere-2024-983

Preprints

15 May 2024

| 15 May 2024

Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water extractable colloids of a Vertisol

Shanshan Bai, Yifei Ge, Dongtan Yao, Yifan Wang, Jinfang Tan, Shuai Zhang, Yutao Peng, and Xiaoqian Jiang

Abstract. Water extractable colloids (WECs) serve as crucial micro particulate components in soils, playing a vital role in the cycling and potential bioavailability of soil phosphorus (P). Yet, the underlying information regarding soil P species and P-transformation microorganisms at the microparticle scale under long-term straw retention and mineral fertilization is barely known. Here, a fixed field experiment (~13 years) in a Vertisol was performed to explore the impacts of straw retention and mineral fertilization on inorganic P, organic P and P-transformation microorganisms in bulk soils and WECs by sequential extraction procedure, P K-edge X-ray absorptions near-edge structure (XANES), ³¹P nuclear magnetic resonance (NMR), and metagenomics analysis. In bulk soil, mineral fertilization led to increases in the levels of total P, available P, acid phosphatase (ACP), high-activity inorganic P fractions (Ca₂-P, Ca₈-P, Al-P, and Fe-P) and organic P (orthophosphate monoesters and orthophosphate diesters), but significantly decreased the abundances of P cycling genes including P mineralization, P-starvation response regulation, P-uptake and transport by decreasing soil pH and increasing P in bulk soil. Straw retention had no significant effects on P species and P-transformation microorganisms in bulk soils but brought increases for organic carbon, total P, available P concentrations in WECs. Furthermore, straw retention caused greater change in P cycling genes between WECs and bulk soils compared with the effect of mineral fertilization. The abundances of phoD gene and phoD-harbouring Proteobacteria in WECs increased significantly under straw retention, suggesting that the P mineralizing capacity increased. Thus, straw retention could potentially accelerate the turnover, mobility and availability of P by increasing the nutrient contents and P mineralizing capacity in microscopic colloidal scale.

Received: 01 Apr 2024 – Discussion started: 15 May 2024

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Journal article(s) based on this preprint

10 Jan 2025

Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol

Shanshan Bai, Yifei Ge, Dongtan Yao, Yifan Wang, Jinfang Tan, Shuai Zhang, Yutao Peng, and Xiaoqian Jiang

Biogeosciences, 22, 135–151, https://doi.org/10.5194/bg-22-135-2025,https://doi.org/10.5194/bg-22-135-2025, 2025

Short summary

Shanshan Bai, Yifei Ge, Dongtan Yao, Yifan Wang, Jinfang Tan, Shuai Zhang, Yutao Peng, and Xiaoqian Jiang

Interactive discussion

Status: closed

RC1:
'Comment on egusphere-2024-983', Anonymous Referee #1, 06 Jun 2024

In this study, a fixed-site field trial was carried out from 2008 to 2021 to examine the impacts of straw (wheat and maize) retention and mineral fertilization (N, P, and K fertilizers) on soil inorganic P fractions, organic P species and P-transformation microorganisms in bulk soils and water-extractable colloid fractions. The paper presented a very exhaustive scientific work, The manuscript represents an important original contribution to research on soil phosphorus dynamics. As evaluation techniques, the methodology used and the results of excellent scientific quality. I recommend being accepted for publication after the minor revision. Please see the specific comments.
1) L141–Please report if some residue was left after digestion.
2) L183–the spectra was not shown in Fig. S4 ?
3) Line 193–There is a misunderstanding in the description. Should be MEGAHIT was used to the assemble genome from reads (fastq formats)
4) Line 208–the normality distribution (Shapiro–Wilks test), not tests?
5) Fig.2– the caption needs to be corrected, What is “the A and B” in these figures?
6) Fig.4–What is “the A and B” in these figures?
7) Table 3– modify the notes, there are three treatments in the tables.
8) Table 5–there are three treatments in the tables.
9) Table S2: glpA, glpB, glpC, glpK should be italic.
10）Fig.S6– Solution ³¹P NMR spectra of NaOH–Na₂EDTA extracts of bulk soil (A) and water-extractable colloids (WECs, B), not bulk soil (a) and water-extractable colloids (WECs, b).

Citation: https://doi.org/10.5194/egusphere-2024-983-RC1
- AC1: 'Reply on RC1', Xiaoqian Jiang, 16 Jul 2024
  
  The comment was uploaded in the form of a supplement: https://egusphere.copernicus.org/preprints/2024/egusphere-2024-983/egusphere-2024-983-AC1-supplement.pdf
  
  Citation: https://doi.org/10.5194/egusphere-2024-983-AC1
RC2:
'Comment on egusphere-2024-983', Anonymous Referee #2, 07 Jun 2024

This paper described the influences of straw retention and mineral fertilizer on the different phosphorous (P) forms in bulk soil and water-extractable colloid fractions, including the two P forms and their specific P specifies. The addition of P-related metagenomics analysis provides a comprehensive map of P cycling concerning different land management in the field. The results are very interesting to the current studies of P cycling in soils, with advanced technologies applied, which can help better understand P transformation in different fractions, but the paper could be more precise and would benefit from restructuring. The statistical methods you chose need to be further considered with respect to your observation size. The Result section is well written in general, but it would be better to modify some of the Figures and clarify some of your results. The major weakness of this paper, from my perspective, is the unclarification of your highlights, which means the Discussion section should be heavily revised. The bullet points in the Discussion section should be clearly delivered accordingly, a summarized paragraph is recommended for each subsection. Therefore, the recommendation for this manuscript is a major revision.
Material and methods
Line 102- Once you decided to use the abbreviation of all your six treatments, use them consistently for the rest of the manuscript
Line 131: Are there reasons why you only fractionate the 3 treatments?
Line 132: Why do you use 'moist soil samples' for sedimentation? Do you also measure the soil texture?
Line 137: I think you mean 'The mass proportion of particles...'
Line 140: Which method and in which soil/solution ratio (w/v) do you use for pH measurement? As far as I know, there are big differences between the CaCl2, H2O2, and KCl methods. And which instrument do you use (specify all the instrument information you use for your analysis)?
Line 140: Any pretreatment for SOC and TN measurements? The chemical measurements need to be briefly described (apply to the rest of the method section e.g. Line 145, Line 155-Line 156...)
Line 169: Reconsider two 'considered'...
Line 207: Specify the version of SPSS and R you use and cite the relevant references. For R, list the packages you use and find the relevant citations.
Line 216: SEM and PCA may not be applicable considering the size of your observation. Please at least check the degree of freedom to see if your interpretation, especially SEM results, can be regarded as reliable and stable. Otherwise, there are also similar models for a smaller observation size.
Results
Line 231: (Table 1) I guess
Line 238: See above, either use the abbreviations or use the full name across the manuscript. And could you explain why you only measured the 3 treatments, not all of them? I also see differences from Table 1 between the 6 treatments and a significant increase in IP fractions, at least in W1M1F1, for example. Otherwise, I would think only include the 3 treatments in your results and discussion.
Line 240: I personally would recommend avoiding overusing unclear words such as 'obvious', 'change', 'effect', 'little' etc., And better avoid 'for example' in the result section. You either present them or not.
Line 283: 'This indicated that...' This, for me, looks like a Discussion, not a Result.
Line 289: 'all the tested samples' make it clear, as you only fractionated 3 treatments, then better not use 'all'
Line 290: 'Staw retention'. which treatment do you mean? sole straw retention?
Line 293: 'The control treatment caused significant...' This also happened in the previous description. The control should perform as a reference, which means it is not a treatment but a benchmark you need to compare firstly with other treatments (e.g. mineral fertilizer, sloe straw retention). The comparisons between other treatments (other than control) can be performed afterwards.
Line 299- Check grammer
Line 310- Does this mean that the other fractions are more sensitive to P cycling genes ? Is it just because of the low proportion of WECs?
Fig. 1: This is quite interesting that straw retention does not increase the OM in total , but only changes the partitioning of OM in different fractions. While, the sole mineral fertilizer significantly increases the OM in total. How do you harvest the plants with sole mineral fertilizer treatment? Do you leave the roots or bottom stems in the field? Missing in the Method
Fig. 2: Increase the text size of the plot and modify the note.
Fig. 4: It is confusing. You tried to compare the two fractions, but it is hard to read the information and make the reader easily think you were comparing the six treatments... and lack of description of A and B. I suggest remaking the figure
Fig. 5: See comments above... Again, you should always compare them with the control, though you want to compare between two fractions...
Fig. 6 See comments above... check degree of freedom
Table 3: There are only 3 treatments in the table
Table 5: There are only 3 treatments in the table
Discussion
The subtitles should be informative and clear, with bullet points emphasized. In addition, all the figures and tables described and shown in the Result section need to be used and properly referenced in the Discussion section; otherwise, the results do not need to be in the Result section. Cite only the necessary literature.
Line 326- 'to the enhanced organic matter from crops...' see comments above, how did you harvest the crops with sole mineral fertilizer input?
Line 342: 'Consistent with our findings...' Normally, it should be 'consistent with previous studies, our study finds...'. The discussion section is to interpret your data and deliver your findings/opinions to the audience instead of describing other studies.
Line 344: 'Long-term P...' is this also mineral P fertilizer?
Line 346-353: Where is your own data? Please reference it and demonstrate it.
Line 359: I assume you tried to compare with the control treatment?
Line 365: If you want to discuss this, then you need to describe it first the CN or CP ratio first in the Result section and reference it in the Discussion section accordingly.
Line 368: Conclued from Table 1?
Line 371: 'The slight increase ...' confused
Line 372: Combine your own data
Line 376: 'Had remarkable influences on...' make it clear, the influence is increase or decrease?
Line 378-379: It is better to have a solid summary based on your data and findings. This finding is not new as the OM decomposition will release organic acid. I expect to see more interesting results from your data.
Line 381: 'AP'?
Line 383: 'The influences of...' Make your opinions clear with referenced results; how do you define the stronger effect?
Line 394: 'slight increase' is it significant?
Line 395: 'considerable influence...' How much is the influence? increase or decrease?
Line 399: 'Research conducted...' It seems these are not related to your context.
Line 410: 'clay particles'? <2 micrometers will be enough, in my opinion.
Line 419: Check the logic framework and how you get to this conclusion.
Line 422: Check the logic framework and how you get to this conclusion.

Concluding remark:

This manuscript has an interesting dataset, while the current structure is not applicable for publication. I hope that a thorough revision can improve the manuscript so that it can meet the criteria for publication. Good luck!

Citation: https://doi.org/10.5194/egusphere-2024-983-RC2
- AC2: 'Reply on RC2', Xiaoqian Jiang, 16 Jul 2024
  
  The comment was uploaded in the form of a supplement: https://egusphere.copernicus.org/preprints/2024/egusphere-2024-983/egusphere-2024-983-AC2-supplement.pdf
  
  Citation: https://doi.org/10.5194/egusphere-2024-983-AC2

Interactive discussion

Status: closed

RC1:
'Comment on egusphere-2024-983', Anonymous Referee #1, 06 Jun 2024

In this study, a fixed-site field trial was carried out from 2008 to 2021 to examine the impacts of straw (wheat and maize) retention and mineral fertilization (N, P, and K fertilizers) on soil inorganic P fractions, organic P species and P-transformation microorganisms in bulk soils and water-extractable colloid fractions. The paper presented a very exhaustive scientific work, The manuscript represents an important original contribution to research on soil phosphorus dynamics. As evaluation techniques, the methodology used and the results of excellent scientific quality. I recommend being accepted for publication after the minor revision. Please see the specific comments.
1) L141–Please report if some residue was left after digestion.
2) L183–the spectra was not shown in Fig. S4 ?
3) Line 193–There is a misunderstanding in the description. Should be MEGAHIT was used to the assemble genome from reads (fastq formats)
4) Line 208–the normality distribution (Shapiro–Wilks test), not tests?
5) Fig.2– the caption needs to be corrected, What is “the A and B” in these figures?
6) Fig.4–What is “the A and B” in these figures?
7) Table 3– modify the notes, there are three treatments in the tables.
8) Table 5–there are three treatments in the tables.
9) Table S2: glpA, glpB, glpC, glpK should be italic.
10）Fig.S6– Solution ³¹P NMR spectra of NaOH–Na₂EDTA extracts of bulk soil (A) and water-extractable colloids (WECs, B), not bulk soil (a) and water-extractable colloids (WECs, b).

Citation: https://doi.org/10.5194/egusphere-2024-983-RC1
- AC1: 'Reply on RC1', Xiaoqian Jiang, 16 Jul 2024
  
  The comment was uploaded in the form of a supplement: https://egusphere.copernicus.org/preprints/2024/egusphere-2024-983/egusphere-2024-983-AC1-supplement.pdf
  
  Citation: https://doi.org/10.5194/egusphere-2024-983-AC1
RC2:
'Comment on egusphere-2024-983', Anonymous Referee #2, 07 Jun 2024

This paper described the influences of straw retention and mineral fertilizer on the different phosphorous (P) forms in bulk soil and water-extractable colloid fractions, including the two P forms and their specific P specifies. The addition of P-related metagenomics analysis provides a comprehensive map of P cycling concerning different land management in the field. The results are very interesting to the current studies of P cycling in soils, with advanced technologies applied, which can help better understand P transformation in different fractions, but the paper could be more precise and would benefit from restructuring. The statistical methods you chose need to be further considered with respect to your observation size. The Result section is well written in general, but it would be better to modify some of the Figures and clarify some of your results. The major weakness of this paper, from my perspective, is the unclarification of your highlights, which means the Discussion section should be heavily revised. The bullet points in the Discussion section should be clearly delivered accordingly, a summarized paragraph is recommended for each subsection. Therefore, the recommendation for this manuscript is a major revision.
Material and methods
Line 102- Once you decided to use the abbreviation of all your six treatments, use them consistently for the rest of the manuscript
Line 131: Are there reasons why you only fractionate the 3 treatments?
Line 132: Why do you use 'moist soil samples' for sedimentation? Do you also measure the soil texture?
Line 137: I think you mean 'The mass proportion of particles...'
Line 140: Which method and in which soil/solution ratio (w/v) do you use for pH measurement? As far as I know, there are big differences between the CaCl2, H2O2, and KCl methods. And which instrument do you use (specify all the instrument information you use for your analysis)?
Line 140: Any pretreatment for SOC and TN measurements? The chemical measurements need to be briefly described (apply to the rest of the method section e.g. Line 145, Line 155-Line 156...)
Line 169: Reconsider two 'considered'...
Line 207: Specify the version of SPSS and R you use and cite the relevant references. For R, list the packages you use and find the relevant citations.
Line 216: SEM and PCA may not be applicable considering the size of your observation. Please at least check the degree of freedom to see if your interpretation, especially SEM results, can be regarded as reliable and stable. Otherwise, there are also similar models for a smaller observation size.
Results
Line 231: (Table 1) I guess
Line 238: See above, either use the abbreviations or use the full name across the manuscript. And could you explain why you only measured the 3 treatments, not all of them? I also see differences from Table 1 between the 6 treatments and a significant increase in IP fractions, at least in W1M1F1, for example. Otherwise, I would think only include the 3 treatments in your results and discussion.
Line 240: I personally would recommend avoiding overusing unclear words such as 'obvious', 'change', 'effect', 'little' etc., And better avoid 'for example' in the result section. You either present them or not.
Line 283: 'This indicated that...' This, for me, looks like a Discussion, not a Result.
Line 289: 'all the tested samples' make it clear, as you only fractionated 3 treatments, then better not use 'all'
Line 290: 'Staw retention'. which treatment do you mean? sole straw retention?
Line 293: 'The control treatment caused significant...' This also happened in the previous description. The control should perform as a reference, which means it is not a treatment but a benchmark you need to compare firstly with other treatments (e.g. mineral fertilizer, sloe straw retention). The comparisons between other treatments (other than control) can be performed afterwards.
Line 299- Check grammer
Line 310- Does this mean that the other fractions are more sensitive to P cycling genes ? Is it just because of the low proportion of WECs?
Fig. 1: This is quite interesting that straw retention does not increase the OM in total , but only changes the partitioning of OM in different fractions. While, the sole mineral fertilizer significantly increases the OM in total. How do you harvest the plants with sole mineral fertilizer treatment? Do you leave the roots or bottom stems in the field? Missing in the Method
Fig. 2: Increase the text size of the plot and modify the note.
Fig. 4: It is confusing. You tried to compare the two fractions, but it is hard to read the information and make the reader easily think you were comparing the six treatments... and lack of description of A and B. I suggest remaking the figure
Fig. 5: See comments above... Again, you should always compare them with the control, though you want to compare between two fractions...
Fig. 6 See comments above... check degree of freedom
Table 3: There are only 3 treatments in the table
Table 5: There are only 3 treatments in the table
Discussion
The subtitles should be informative and clear, with bullet points emphasized. In addition, all the figures and tables described and shown in the Result section need to be used and properly referenced in the Discussion section; otherwise, the results do not need to be in the Result section. Cite only the necessary literature.
Line 326- 'to the enhanced organic matter from crops...' see comments above, how did you harvest the crops with sole mineral fertilizer input?
Line 342: 'Consistent with our findings...' Normally, it should be 'consistent with previous studies, our study finds...'. The discussion section is to interpret your data and deliver your findings/opinions to the audience instead of describing other studies.
Line 344: 'Long-term P...' is this also mineral P fertilizer?
Line 346-353: Where is your own data? Please reference it and demonstrate it.
Line 359: I assume you tried to compare with the control treatment?
Line 365: If you want to discuss this, then you need to describe it first the CN or CP ratio first in the Result section and reference it in the Discussion section accordingly.
Line 368: Conclued from Table 1?
Line 371: 'The slight increase ...' confused
Line 372: Combine your own data
Line 376: 'Had remarkable influences on...' make it clear, the influence is increase or decrease?
Line 378-379: It is better to have a solid summary based on your data and findings. This finding is not new as the OM decomposition will release organic acid. I expect to see more interesting results from your data.
Line 381: 'AP'?
Line 383: 'The influences of...' Make your opinions clear with referenced results; how do you define the stronger effect?
Line 394: 'slight increase' is it significant?
Line 395: 'considerable influence...' How much is the influence? increase or decrease?
Line 399: 'Research conducted...' It seems these are not related to your context.
Line 410: 'clay particles'? <2 micrometers will be enough, in my opinion.
Line 419: Check the logic framework and how you get to this conclusion.
Line 422: Check the logic framework and how you get to this conclusion.

Concluding remark:

This manuscript has an interesting dataset, while the current structure is not applicable for publication. I hope that a thorough revision can improve the manuscript so that it can meet the criteria for publication. Good luck!

Citation: https://doi.org/10.5194/egusphere-2024-983-RC2
- AC2: 'Reply on RC2', Xiaoqian Jiang, 16 Jul 2024
  
  The comment was uploaded in the form of a supplement: https://egusphere.copernicus.org/preprints/2024/egusphere-2024-983/egusphere-2024-983-AC2-supplement.pdf
  
  Citation: https://doi.org/10.5194/egusphere-2024-983-AC2

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision | EF: Editorial file upload

ED: Reconsider after major revisions (17 Jul 2024) by Lucia Fuchslueger

AR by Xiaoqian Jiang on behalf of the Authors (21 Jul 2024) Author's response Author's tracked changes Manuscript

ED: Referee Nomination & Report Request started (01 Aug 2024) by Lucia Fuchslueger

RR by Anonymous Referee #2 (12 Aug 2024)

Suggestions for revision or reasons for rejection

This revised manuscript has significantly improved, but many minor construction mistakes and grammar issues need to be carefully and thoroughly revised. Therefore, the recommendation for this manuscript at this stage is a minor revision.
Abstract
Line 26: increasing which P? Total P I guess?
Line 29: Could you please specify ‘change’?
Line 31: It will be better to add one summary sentence. ‘Thus, …’ only contains the results from straw retention, lack of context regarding to the mineral fertilization as you mentioned in the title
Material and Methods
Line 146: If you decide to use abbreviations like SOC, TN here, you need to clarify them at the beginning of your manuscript and use the abbreviations constantly throughout the manuscript. In the abstract as well.
Line 155: Abbreviation? Check throughout the whole manuscript.
Line 183: Organic P species?
Discussion
The subtitles are informative. The construction of the discussion has been remarkedly improved. But there are still many minor grammar and logical issues. Fig.2 and Fig. 4 need to be recognizable to readers, so I suggest greatly increasing the font size.
Line 333-334: For me, ‘mineral fertilization decreased soil pH, increase soil TP, thus decreasing the …’ has a logical issue. I think these are parallel results obtained from Table 1 and Fig. 2, the causal relationship needs correlation results like Fig. 6.
Line 338: Not relevant to your core topic
Line 407: See comments above.
Line 346-353: Where is your own data? Please reference it and demonstrate it.
Conclusion
The conclusion section should contain the clear main results the same as the Abstract. The summarized paragraphs at the end of each Discussion part can be summarized here. Meanwhile, the conclusion should explain the contribution of your research to the knowledge gap you introduced at the start of the abstract and introduction, and some perspectives if there are any.

Hide

ED: Publish subject to minor revisions (review by editor) (06 Sep 2024) by Lucia Fuchslueger

AR by Xiaoqian Jiang on behalf of the Authors (17 Sep 2024) Author's response Author's tracked changes Manuscript

ED: Publish subject to minor revisions (review by editor) (05 Oct 2024) by Lucia Fuchslueger

Dear authors,

thank you for considering and following the reviewer's suggestions, in particular to improve the Figures. However, I would request some further changes, and as also already previously pointed out by the reviewers earlier, I would recommend to have some quick language check.
Moreover, please also add some more details in the methods sections (some details are specified below in the line-by-line section). Please pay particular attention especially on the section 2.6 DNA extraction and metagenomics analysis. The details provided are still quite vague in some sections and it would be important to add more technical details. Please mention how the reads were filtered, and provide details on the pre-processing steps and a brief statement with details on the Diamond settings that have been used.
Finally, I would highly encourage to restructure the conclusions sections. At the moment the mostly summarize the results, only the very last sentence is rather a conclusion. To make the manuscript stronger, I would recommend that the authors could rephrase and rather focus on impacts or implications of the outcomes of their study.

Below are some points I would like you to resolve specifically:
Line 26: remove the last 'in bulk soil'.
Line 28: change to: ', but lead to increases in..'
Line 29: this sentence is not really easy to understand, which treatment caused higher P cycling gene abundance compared to which?
Line 47: when you use the Ca-P, Al-P etc... please first time spell them out or explain a bit better.
Line 63: delete the space before 0.25
Line 91: this first sentence is a bit out of context here. Maybe change to:
In a long-term (13 year) field experiment modulating straw retention and mineral fertilization, we investigated 1) the responses....
Line 95: This sentences could be removed, as they are almost repeating the same message as just before the research aim (Lines 89-90)
Line 124: Change to: From all six treatment plots soil samples were collected after wheat harvest in June 2021
Line 128: delete 'the' before 'acid and alkaline phosphatase'
Line 151: please add here, to remove inorganic carbon (and change stop to stopped).
Line 154: change to ‘using the method described by Olsen...’
Line 155: leave the abbreviation in brackets: dissolved organic carbon (DOC)...
Line 160 and 162: provide references for the conversion factor
LIne 200: change to: DNA was extracted using a Fast...
Line 233: change 'agricultural managements' to 'agricultural management types'
Line 238: change 'incorporated' to 'in combination with'
Line 239: better mention the values changed from pH x to y than the differences
Line 240 and throughout the manuscript: use SI-units e.g. g kg-1 or µg g-1 h-1
Line 243: change to: there was not significant effect of straw retention detectable
Line 247-250: this is very vague, can this turned more specific maybe?
Line 279: remove ‘that’ after manifested
Line 283: remove ‘the’ before samples
Line 286: stick to uniform tenses
Line 316: remove ‘the’ before treatments
Line 322: this is not a full sentence, please modify (whereas at the begin of the sentence needs to be changed).
Line 390: ... I suggest to change to: could be caused by the higher specific surface area of the WEC
Line 391 and general, some sentences are very long and could be shortened

Hide

AR by Xiaoqian Jiang on behalf of the Authors (22 Oct 2024) Author's response Author's tracked changes Manuscript

ED: Publish as is (31 Oct 2024) by Lucia Fuchslueger

AR by Xiaoqian Jiang on behalf of the Authors (09 Nov 2024)

Journal article(s) based on this preprint

10 Jan 2025

Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol

Shanshan Bai, Yifei Ge, Dongtan Yao, Yifan Wang, Jinfang Tan, Shuai Zhang, Yutao Peng, and Xiaoqian Jiang

Biogeosciences, 22, 135–151, https://doi.org/10.5194/bg-22-135-2025,https://doi.org/10.5194/bg-22-135-2025, 2025

Short summary

Shanshan Bai, Yifei Ge, Dongtan Yao, Yifan Wang, Jinfang Tan, Shuai Zhang, Yutao Peng, and Xiaoqian Jiang

Supplement

https://doi.org/10.5194/egusphere-2024-983-supplement

Shanshan Bai, Yifei Ge, Dongtan Yao, Yifan Wang, Jinfang Tan, Shuai Zhang, Yutao Peng, and Xiaoqian Jiang

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Short summary

Mineral fertilization led to increases in total P, available P, high-activity inorganic P fractions and organic P, but decreased the abundances of P cycling genes by decreasing soil pH and increasing P in bulk soil. Straw retention brought increases for organic C, total P, available P concentrations in water-extractable colloids (WECs). Abundances of phoD gene and phoD-harbouring Proteobacteria in WECs increased under straw retention, suggesting that the P mineralizing capacity increased.


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