Old Carbon, New Insights: Thermal Reactivity and Bioavailability of Saltmarsh Soils

Houston, Alex; Garnett, Mark H.; Smith, Jo; Austin, William E. N.

doi:https://doi.org/10.5194/egusphere-2024-3281

Preprints

https://doi.org/10.5194/egusphere-2024-3281

Preprints

13 Nov 2024

| 13 Nov 2024

Old Carbon, New Insights: Thermal Reactivity and Bioavailability of Saltmarsh Soils

Alex Houston, Mark H. Garnett, Jo Smith, and William E. N. Austin

Abstract. Saltmarshes are globally important coastal wetlands which can store carbon for millennia, helping to mitigate the impacts of climate change. They accumulate organic carbon from both autochthonous sources (above- and belowground plant production) and allochthonous sources (terrestrial and marine sediments deposited during tidal inundation). Previous studies have found that long-term organic carbon storage in saltmarsh soils is driven by the pre-aged allochthonous fraction, implying that autochthonous organic carbon is recycled at a faster rate. However, it is also acknowledged that the bioavailability of soil organic carbon depends as much upon environmental conditions as the reactivity of the organic carbon itself. Until now, there has been no empirical evidence linking the reactivity of saltmarsh soil organic carbon with its bioavailability for remineralization.

We found that the ¹⁴C age of CO₂ produced during ramped oxidation of soils from the same saltmarsh ranged from 201 to 14,875 years BP, and that ¹⁴C-depleted (older) carbon evolved from higher temperature ramped oxidation fractions, indicating that older carbon dominates the thermally recalcitrant fractions. In most cases, the ¹⁴C content of the lowest temperature ramped oxidation fraction (the most thermally labile organic C source) was closest to the previously reported ¹⁴C content of the CO₂ evolved from aerobic incubations of the same soils, implying that the latter was from a thermally labile organic carbon source. This implies that the bioavailability of saltmarsh soil organic carbon to remineralisation in oxic conditions is closely related to its thermal reactivity. Management interventions (e.g. rewetting by tidal inundation) to limit the exposure of saltmarsh soils to elevated oxygen availability may help to protect and conserve these stores of old, labile organic carbon and hence limit CO₂ emissions.

Received: 21 Oct 2024 – Discussion started: 13 Nov 2024

Publisher's note: Copernicus Publications remains neutral with regard to jurisdictional claims made in the text, published maps, institutional affiliations, or any other geographical representation in this paper. While Copernicus Publications makes every effort to include appropriate place names, the final responsibility lies with the authors. Views expressed in the text are those of the authors and do not necessarily reflect the views of the publisher.

Download & links

Preprint (PDF, 987 KB)

Supplement (9664 KB)

Download & links

Alex Houston, Mark H. Garnett, Jo Smith, and William E. N. Austin

Status: closed

RC1:
'Comment on egusphere-2024-3281', Jordon Hemingway, 18 Dec 2024

Please find my review in the attached pdf file.

Citation: https://doi.org/10.5194/egusphere-2024-3281-RC1
- AC1:
  'Reply on RC1', Alex Houston, 17 Jan 2025
  We thank the reviewer for their focused and detailed review of our manuscript. We appreciate the constructive feedback and the opportunity to improve this manuscript. We welcome the comment that the topic of this manuscript is an interesting and underexplored area of research. In this response letter we will address the key points of the review, while stopping short of providing a revised manuscript until a formal editorial decision is made.
  Primary R(P)O literature and context
  
  We agree with the reviewer’s comment that there is insufficient citing of the primary ramped (pyrolysis) oxidation (R(P)O) literature and thank them for sharing a list of sources to potentially include. This issue stems from an attempt to keep the manuscript concise, however, we acknowledge that this has resulted in some of the primary RO literature being excluded or not covered in sufficient detail. We will cite these more extensively in the revised manuscript.
  RO data analysis and interpretation
  
  We acknowledge that calculating activation energies could improve the interpretation of the data as the reviewer suggests and welcome the suggestion to utilise their ‘rampedpyrox’ package. However, we believe that the novel research questions that this manuscript sets out to answer do not require the calculation of activation energies, given the existence of other published RO literature which do not calculate these and make the same inferences about temperature and thermal reactivity as we do (e.g. Grant et al., 2019; Peltre et al., 2013; Sanderman and Grandy, 2020; Stoner et al., 2023; Williams et al., 2018).
  We initially understood that this model would not be suitable for our samples as they have not been decarbonated which is stated as a requirement (Hemingway et al., 2017). Therefore, we proceeded with inferring thermal reactivity from the chosen temperature intervals in the submitted manuscript.
  Hemingway et al., 2017; P3: “the presence of carbonate will result in thermograms that cannot be accurately described by the model presented here, and we therefore argue in favor of acid treatment when using the RPO instrument to determine reaction energetics of carbonate-containing samples.”
  Following receipt of this review, we contacted the reviewer to determine if the ‘rampexpyrox’ model was potentially suitable for our samples, despite the above published advice that they were not. The reviewer has now confirmed (pers. comm. 16/01/2025) that due to the low amount of carbonates in our samples that it would be worth attempting to calculate activation energies. We will therefore implement the ‘rampedpyrox’ model for analysis and presentation of the thermograms and isotope data in the revised manuscript; we thank the reviewer for this clarification.
  Regarding the normalisation of thermograms to maximum peak size, this is simply to aid visual comparison of the curves between the samples. The regressions were not based on the normalised curves, but on the volume of CO₂ evolved over the defined temperature intervals.
  As we will be implementing the ‘rampedpyrox’ model for our data analysis and presentation of the thermograms and isotope data, the figures used in the results section will be updated in the revised manuscript. Therefore, the normalised thermograms and regressions presented in the initial manuscript will likely be removed from the revised version and replaced by a discussion of the distribution of activation energies (and related ¹⁴C contents).
  Analysis and measurement detail
  
  A published paper verifying the RO methodology used in this manuscript already exists (Garnett et al., 2023) and is cited in this paper. We will add the required details such as carrier gas composition, sample masses, and flow rate to the revised manuscript. We thank the reviewer for their suggested verification checks and we will add the mass balance verifications to the revised manuscript where possible.
  The decision to not remove carbonates from our samples was taken to avoid the potential loss of carbon from labile fractions during acid treatment (Bao et al., 2019). Importantly, we note that in Hemingway et al. (2017), acid treatment of samples prior to RO resulted in a shift of 0.04 Fm ¹⁴C. This effect could shift one of our samples from having a pre-bomb ¹⁴C content to a post-bomb ¹⁴C content, or vice-versa. A similar shift in ¹⁴C content for our samples could seriously impact the interpretations in our study, and our ability to compare the ¹⁴C content of the CO₂ respired from bulk (untreated) soils in the incubation experiments (Houston et al., 2024) to the ¹⁴C content of the CO₂ evolved during the RO procedure. This is a crucial component of this work and hence why samples were not decarbonated.
  Furthermore, the detail on the treatment of these samples is stated in the manuscript, L109: “The RO sub-samples were individually dried to constant mass before milling to a fine powder to homogenise and limit potential shielding effects from aggregates. The samples were then sent to the NEIF Radiocarbon Laboratory for the RO procedure, which is described in Garnett et al. (2023)”. We acknowledge the fact that we did not acid-treat our samples is implicit rather than explicitly stated, so we will add in this detail to the revised manuscript to avoid confusing future readers. We will also add a discussion section detailing why we did not decarbonate our samples and will also report the inorganic carbon content of the bulk soil samples in the supplementary information (these are <0.5 % inorganic carbon). In this new section we will also discuss how not decarbonating our samples may have impacted our results and interpretations.
  Regarding the normalisation of ¹⁴C values, it is incorrect to state that using δ¹³C values (determined using isotope ratio mass spectrometry (IRMS) from an aliquot of the sample CO₂) to normalise ¹⁴C results represents a major break from typical procedure. Use of IRMS δ¹³C values for normalising AMS data is clearly supported in several key AMS methods papers (Donahue et al., 1990; McNichol et al., 2001) and while online AMS ¹³C measurements can be advantageous particularly for small samples (Santos et al., 2007), many labs employ IRMS values for normalisation (McIntyre et al., 2017) and quality assurance results show that the method is clearly reliable (Ascough et al., 2024).
  Data validation and verification
  
  The RO samples are sub-sets of those analysed in Houston et al. (2024), where the bulk sample %OC, %IC, pMC, and δ¹³C values are all reported (Houston et al., 2024). To aid comparison for readers, we will add these into the supplementary material in the revised manuscript. We will also add sense-checks such as isotope mass-balance calculations to the supplementary material.
  We welcome the reviewer’s point about the potential (or lack thereof) for ¹³C fractionation during the RO procedure. We will alter this section of the manuscript and discuss in greater detail the mechanisms such as different compounds combusting at different temperature ranges and the importance of organo-mineral interactions for some compound classes. However, ¹⁴C is the main focus of this manuscript (comparison of ¹⁴C content of respired CO₂ from incubation experiments and ¹⁴C content of thermal reactivity fractions).
  We will also discuss the potential for carbonate contribution as low as ~550 °C here. We note that the ¹³C values for each of the 500-650 °C RO fractions were within expected ranges for OC in these samples, but that there could be contributions from carbonate sources. As previously stated, we will be implementing the ‘rampedpyrox’ model for our data analysis and presentation of the thermograms and isotope data, so the interpretations of ¹⁴C content and temperature will be replaced in the revised manuscript by comparing ¹⁴C contents of the evolved CO₂ to activation energies.
  Concepts and terminology
  
  We will improve on our terminology usage in the revised manuscript and thank the reviewer for their suggested terms of “thermal recalcitrance” and “thermal lability” which we will use when referring to the thermal reactivity of the OC pools. We will retain the use of “bioavailable OC” when referring to the CO₂ respired from the incubation experiments. We agree with the reviewer that it is important to highlight to the reader that thermal reactivity does not have to correlate with biological turnover times. Interestingly, this was one of the motivations for undertaking this work as much previous work has been undertaken on the ‘reactivity’ of soil/sediment OC but there has been limited work comparing this to ‘bioavailable’ carbon under set environmental conditions.
  We take on board the reviewer’s comments of not using ¹⁴C ‘ages’. We had included this to provide context to the most ¹⁴C-depleted samples to highlight the potential for ¹⁴C-dead contributions but that they aren’t necessary to achieve these low ¹⁴C contents for the region. However, we will remove this interpretation in the revised manuscript to avoid inappropriate reporting of complex OC mixtures.
  Regressions, plotting, and statistics
  
  We agree with the reviewer that the regressions are not ideal as they are calculated from the mean temperature, but we now acknowledge that the ¹⁴C (or ¹³C) content could be predominantly evolved from a narrower temperature interval within the defined temperature range of CO2 collection. However, we agree with the reviewer that these plots could be clearer and welcome their data visualisation suggestions which we will implement in the revised manuscript.
  Additionally, we agree that the box and whisker plots result in a loss of crucial information about individual samples.
  The omission of the 650-800 °C RO fraction from Figure 3 was to compare the OC pools. However, we strongly disagree with the reviewer that this was a dishonest omission as the δ¹³C for the 650-800 °C RO fraction are reported in Table 2, directly below this figure.
  As we will be implementing the ‘rampedpyrox’ model for our data analysis and presentation of the thermograms and isotope data a lot of the figures used in the results section will be updated in the revised manuscript.
  We agree with the reviewer that we should remove any interpretation of statistically insignificant trends from the revised manuscript.
  
  Yours sincerely,
  Alex Houston (on behalf of all authors).
  
  Reference List
  Ascough, P., Bompard, N., Garnett, M. H., Gulliver, P., Murray, C., Newton, J.-A., and Taylor, C.: 14C MEASUREMENT OF SAMPLES FOR ENVIRONMENTAL SCIENCE APPLICATIONS AT THE NATIONAL ENVIRONMENTAL ISOTOPE FACILITY (NEIF) RADIOCARBON LABORATORY, SUERC, UK, Radiocarbon, 66, 1020–1031, https://doi.org/10.1017/RDC.2024.9, 2024.
  Bao, R., McNichol, A. P., Hemingway, J. D., Gaylord, M. C. L., and Eglinton, T. I.: Influence of Different Acid Treatments on the Radiocarbon Content Spectrum of Sedimentary Organic Matter Determined by RPO/Accelerator Mass Spectrometry, Radiocarbon, 61, 395–413, https://doi.org/10.1017/RDC.2018.125, 2019.
  Dasari, S., Garnett, M. H., and Hilton, R. G.: Leakage of old carbon dioxide from a major river system in the Canadian Arctic, PNAS Nexus, 3, pgae134, https://doi.org/10.1093/pnasnexus/pgae134, 2024.
  Dean, J. F., Billett, M. F., Turner, T. E., Garnett, M. H., Andersen, R., McKenzie, R. M., Dinsmore, K. J., Baird, A. J., Chapman, P. J., and Holden, J.: Peatland pools are tightly coupled to the contemporary carbon cycle, Global Change Biology, n/a, e16999, https://doi.org/10.1111/gcb.16999, 2023.
  Donahue, D. J., Linick, T. W., and Jull, A. J. T.: Isotope-Ratio and Background Corrections for Accelerator Mass Spectrometry Radiocarbon Measurements, Radiocarbon, 32, 135–142, https://doi.org/10.1017/S0033822200040121, 1990.
  Garnett, M. H. and Hardie, S. M. L.: Isotope (14C and 13C) analysis of deep peat CO2 using a passive sampling technique, Soil Biology and Biochemistry, 41, 2477–2483, https://doi.org/10.1016/j.soilbio.2009.09.004, 2009.
  Garnett, M. H., Hardie, S. M. L., and Murray, C.: Radiocarbon analysis reveals that vegetation facilitates the release of old methane in a temperate raised bog, Biogeochemistry, 148, 1–17, https://doi.org/10.1007/s10533-020-00638-x, 2020.
  Garnett, M. H., Pereira, R., Taylor, C., Murray, C., and Ascough, P. L.: A NEW RAMPED OXIDATION-14C ANALYSIS FACILITY AT THE NEIF RADIOCARBON LABORATORY, EAST KILBRIDE, UK, Radiocarbon, 65, 1213–1229, https://doi.org/10.1017/RDC.2023.96, 2023.
  Grant, K. E., Galy, V. V., Chadwick, O. A., and Derry, L. A.: Thermal oxidation of carbon in organic matter rich volcanic soils: insights into SOC age differentiation and mineral stabilization, Biogeochemistry, 144, 291–304, https://doi.org/10.1007/s10533-019-00586-1, 2019.
  Hemingway, J. D., Rothman, D. H., Rosengard, S. Z., and Galy, V. V.: Technical note: An inverse method to relate organic carbon reactivity to isotope composition from serial oxidation, Biogeosciences, 14, 5099–5114, https://doi.org/10.5194/bg-14-5099-2017, 2017.
  Houston, A., Garnett, M. H., and Austin, W. E. N.: Blue carbon additionality: New insights from the radiocarbon content of saltmarsh soils and their respired CO2, Limnology and Oceanography, n/a, https://doi.org/10.1002/lno.12508, 2024.
  McIntyre, C. P., Wacker, L., Haghipour, N., Blattmann, T. M., Fahrni, S., Usman, M., Eglinton, T. I., and Synal, H.-A.: Online 13C and 14C Gas Measurements by EA-IRMS–AMS at ETH Zürich, Radiocarbon, 59, 893–903, https://doi.org/10.1017/RDC.2016.68, 2017.
  McNichol, A. P., Jull, A. J. T., and Burr, G. S.: Converting AMS Data to Radiocarbon Values: Considerations and Conventions, Radiocarbon, 43, 313–320, https://doi.org/10.1017/S0033822200038169, 2001.
  Peltre, C., Fernández, J. M., Craine, J. M., and Plante, A. F.: Relationships between Biological and Thermal Indices of Soil Organic Matter Stability Differ with Soil Organic Carbon Level, Soil Science Society of America Journal, 77, 2020–2028, https://doi.org/10.2136/sssaj2013.02.0081, 2013.
  Sanderman, J. and Grandy, A. S.: Ramped thermal analysis for isolating biologically meaningful soil organic matter fractions with distinct residence times, SOIL, 6, 131–144, https://doi.org/10.5194/soil-6-131-2020, 2020.
  Santos, G. M., Moore, R. B., Southon, J. R., Griffin, S., Hinger, E., and Zhang, D.: AMS 14C Sample Preparation at the KCCAMS/UCI Facility: Status Report and Performance of Small Samples, Radiocarbon, 49, 255–269, https://doi.org/10.1017/S0033822200042181, 2007.
  Stoner, S. W., Schrumpf, M., Hoyt, A., Sierra, C. A., Doetterl, S., Galy, V., and Trumbore, S.: How well does ramped thermal oxidation quantify the age distribution of soil carbon? Assessing thermal stability of physically and chemically fractionated soil organic matter, Biogeosciences, 20, 3151–3163, https://doi.org/10.5194/bg-20-3151-2023, 2023.
  Williams, E. K., Fogel, M. L., Berhe, A. A., and Plante, A. F.: Distinct bioenergetic signatures in particulate versus mineral-associated soil organic matter, Geoderma, 330, 107–116, https://doi.org/10.1016/j.geoderma.2018.05.024, 2018.
  
  Citation: https://doi.org/10.5194/egusphere-2024-3281-AC1
RC2:
'Comment on egusphere-2024-3281', Anonymous Referee #2, 17 Mar 2025

General Comments:
This is a an important piece of work investigating the reactivity of saltmarsh soil organic carbon. The study found that 14C-depleted (older) carbon evolved from higher temperature ramped oxidation fractions, indicating that older carbon dominates the thermally recalcitrant fractions. The work does progress the field and offers new insights into our understanding of the composition of this important carbon sink. However, there are a few concerns with the manuscript that require some consideration and revision.

Specific Comments
The use of the term “pre-aged” (especially in the abstract) is ambiguous and should be clarified to the reader without needing to go back to Houston et al (2024).
In the methods section there should be a sub section on data analysis. What programmes were used, how was the data treated?
Also Line 105-106. “the deepest sample from each core being the deepest retrieved sample” – does this mean that the depth to refusal at this site is 20cm?
As regards the results section – the main query is the categorical approach to representing the data in Figure 2 and Figure 3 and using the exponential and linear regression (respectively). The categorical approach can stand alone without the regresssions, however if using regressions then the x axis should be represented as a continuous variable. Please consider revising this.
In terms of the two tables in the main body of the manuscript -is it possible to graphically represent some of this data? I think much like the graphical abstract, there is scope to conceptualize the data in diagrammatic form to increase the impact of the findings.
Is there data or any published work on the carbon accumulation rates at this site? How do the authors think the findings of this study would integrate with C org accumulation rate data.
As these samples are taken from the lower shore of the saltmarsh site can the authors speculate on how the results could vary with proximity to the terrestrial border and the greater proportion of organic carbon from autochthonous sources?
The abstract concludes with a sentence on how this study has relevance for saltmarsh management and therefore I expected a portion of the discussion to focus on this but I found this was just briefly mentioned at the end of the discussion and conclusion sections. I think greater discussion of the practical implications that the findings may have is necessary.

Citation: https://doi.org/10.5194/egusphere-2024-3281-RC2
- AC2: 'Reply on RC2', Alex Houston, 25 Mar 2025
  
  We thank Reviewer 2 for their constructive and helpful comments on our manuscript, and for their highlighting of the importance and novelty of this research:
  “This is an important piece of work investigating the reactivity of saltmarsh soil organic carbon. The study found that 14C-depleted (older) carbon evolved from higher temperature ramped oxidation fractions, indicating that older carbon dominates the thermally recalcitrant fractions. The work does progress the field and offers new insights into our understanding of the composition of this important carbon sink.”
  Below, we outline our response to each of their specific comments.
  The use of the term “pre-aged” (especially in the abstract) is ambiguous and should be clarified to the reader without needing to go back to Houston et al (2024).
  In a revised manuscript, we will change ‘pre-aged’ to ‘aged’ to clarify for the reader that the input of organic matter to blue carbon soils need not always be the same age as the in-situ production of plant material.
  In the methods section there should be a sub section on data analysis. What programmes were used, how was the data treated?
  We will add a ‘data analysis’ section to a revised manuscript. In the current version of this manuscript all data analysis and visualisation of thermograms and isotopic data was undertaken using RStudio V4.2.2. In a revised manuscript, we will use Python V3.8 to implement the ‘rampedpyrox’ package to calculate activation energy distributions for our samples from the thermograms, as also requested by Reviewer 1 (Hemingway, 2016; Hemingway et al., 2017)
  Also Line 105-106. “the deepest sample from each core being the deepest retrieved sample” – does this mean that the depth to refusal at this site is 20cm?
  No, in this study we used a golf-hole corer and its length is 20 cm. The depth to refusal at this site is >20 cm (see Miller et al., 2023), and variable across the site. In the revised manuscript we will add an explicit statement about the length of the coring device at the appropriate point in the text and this will add the necessary clarification.
  As regards the results section – the main query is the categorical approach to representing the data in Figure 2 and Figure 3 and using the exponential and linear regression (respectively). The categorical approach can stand alone without the regressions, however if using regressions then the x axis should be represented as a continuous variable. Please consider revising this.
  In the revised manuscript we will implement the ‘rampedpyrox’ package (Hemingway, 2016), based on suggestions from Reviewer 1 (we direct readers to our detailed online response letter to Reviewer 1). This package allows the calculation of continuous activation energy distributions for each ramped oxidation sample, instead of the categorical temperature binning approach we employed in the current version of the manuscript. With this new approach, we will be able to use regressions appropriately.
  In terms of the two tables in the main body of the manuscript -is it possible to graphically represent some of this data? I think much like the graphical abstract, there is scope to conceptualize the data in diagrammatic form to increase the impact of the findings.
  The data from these two tables is presented in this version of the manuscript in Figures 2 and 3. However, in the revised manuscript we will seek to improve our data visualisation to increase the impact of our findings.
  Is there data or any published work on the carbon accumulation rates at this site? How do the authors think the findings of this study would integrate with C org accumulation rate data.
  Yes, there are published carbon (C) accumulation rates for this site (Miller et al., 2023; Smeaton et al., 2024). Compared to other Scottish and UK saltmarshes, Skinflats has relatively high C accumulation rates ( Miller et al., 2023; Smeaton et al., 2024). In previously published work we showed that Skinflats accumulates carbon of a much greater ‘age’ (depleted soil ¹⁴C contents) than two other saltmarshes in Scotland (Houston et al., 2024). The combination of high carbon accumulation rates and depleted soil ¹⁴C contents implies that the Skinflats saltmarsh accumulates a high proportion of aged, most likely allochthonous C. This is potentially why we don’t see a clear decrease in the thermally labile ramped oxidation fractions with depth in this study - as much of the accumulated C has already been processed/degraded prior to deposition at the core site. Our findings in this study, that aged C has a greater thermal recalcitrance than young C, imply that sites accumulating younger C would have more thermally labile soil C. We will add detail on these interpretations and potential implications to the discussion section of the revised manuscript.
  As these samples are taken from the lower shore of the saltmarsh site can the authors speculate on how the results could vary with proximity to the terrestrial border and the greater proportion of organic carbon from autochthonous sources?
  We will add detail on this to the revised manuscript. Given our findings that more aged carbon has greater thermal recalcitrance, we anticipate that higher marsh zones, which typically have greater proportions of autochthonous C than lower marsh zones, would have a greater proportion of thermally labile C. However, it is important to recognise that young, autochthonous C can also be thermally recalcitrant. As well as with marsh zone, we expect that the proportion of C sources and thermal reactivity would vary with proximity to marsh creeks which redistribute autochthonous and allochthonous C around the site.
  Some recent work, sampling surface sediments across the saltmarsh-mudflat ecotone, has also provided preliminary evidence that low marsh zones (like in this study) have soils which store organic matter of a greater thermal reactivity than adjacent mudflat sediments (McIntosh et al., 2025).
  The abstract concludes with a sentence on how this study has relevance for saltmarsh management and therefore I expected a portion of the discussion to focus on this but I found this was just briefly mentioned at the end of the discussion and conclusion sections. I think greater discussion of the practical implications that the findings may have is necessary.
  We welcome the reviewer’s encouragement to expand on the implications of our results and will add this to the discussion section of the revised manuscript.
  
  Yours sincerely,
  Alex Houston (on behalf of all authors).
  
  Reference List
  Hemingway, J. D.: rampedpyrox: Open-source tools for thermoanalytical data analysis, 2016-, 2016.
  Hemingway, J. D., Rothman, D. H., Rosengard, S. Z., and Galy, V. V.: Technical note: An inverse method to relate organic carbon reactivity to isotope composition from serial oxidation, Biogeosciences, 14, 5099–5114, https://doi.org/10.5194/bg-14-5099-2017, 2017.
  Houston, A., Garnett, M. H., and Austin, W. E. N.: Blue carbon additionality: New insights from the radiocarbon content of saltmarsh soils and their respired CO2, Limnology and Oceanography, n/a, https://doi.org/10.1002/lno.12508, 2024.
  McIntosh, C. L., Smeaton, C., Houston, A., Chalmers, K., Carl, L., Yang, H., and Austin, W. E. N.: Soil organic carbon content and reactivity at the pioneer saltmarsh-mudflat ecotone, Copernicus Meetings, https://doi.org/10.5194/egusphere-egu25-9591, 2025.
  Miller, L. C., Smeaton, C., Yang, H., and Austin, W. E. N.: Carbon accumulation and storage across contrasting saltmarshes of Scotland, Estuarine, Coastal and Shelf Science, 282, 108223, https://doi.org/10.1016/j.ecss.2023.108223, 2023.
  Smeaton, C., Garrett, E., Koot, M. B., Ladd, C. J. T., Miller, L. C., McMahon, L., Foster, B., Barlow, N. L. M., Blake, W., Gehrels, W. R., Skov, M. W., and Austin, W. E. N.: Organic carbon accumulation in British saltmarshes, Science of The Total Environment, 926, 172104, https://doi.org/10.1016/j.scitotenv.2024.172104, 2024.
  
  Citation: https://doi.org/10.5194/egusphere-2024-3281-AC2

Status: closed

RC1:
'Comment on egusphere-2024-3281', Jordon Hemingway, 18 Dec 2024

Please find my review in the attached pdf file.

Citation: https://doi.org/10.5194/egusphere-2024-3281-RC1
- AC1:
  'Reply on RC1', Alex Houston, 17 Jan 2025
  We thank the reviewer for their focused and detailed review of our manuscript. We appreciate the constructive feedback and the opportunity to improve this manuscript. We welcome the comment that the topic of this manuscript is an interesting and underexplored area of research. In this response letter we will address the key points of the review, while stopping short of providing a revised manuscript until a formal editorial decision is made.
  Primary R(P)O literature and context
  
  We agree with the reviewer’s comment that there is insufficient citing of the primary ramped (pyrolysis) oxidation (R(P)O) literature and thank them for sharing a list of sources to potentially include. This issue stems from an attempt to keep the manuscript concise, however, we acknowledge that this has resulted in some of the primary RO literature being excluded or not covered in sufficient detail. We will cite these more extensively in the revised manuscript.
  RO data analysis and interpretation
  
  We acknowledge that calculating activation energies could improve the interpretation of the data as the reviewer suggests and welcome the suggestion to utilise their ‘rampedpyrox’ package. However, we believe that the novel research questions that this manuscript sets out to answer do not require the calculation of activation energies, given the existence of other published RO literature which do not calculate these and make the same inferences about temperature and thermal reactivity as we do (e.g. Grant et al., 2019; Peltre et al., 2013; Sanderman and Grandy, 2020; Stoner et al., 2023; Williams et al., 2018).
  We initially understood that this model would not be suitable for our samples as they have not been decarbonated which is stated as a requirement (Hemingway et al., 2017). Therefore, we proceeded with inferring thermal reactivity from the chosen temperature intervals in the submitted manuscript.
  Hemingway et al., 2017; P3: “the presence of carbonate will result in thermograms that cannot be accurately described by the model presented here, and we therefore argue in favor of acid treatment when using the RPO instrument to determine reaction energetics of carbonate-containing samples.”
  Following receipt of this review, we contacted the reviewer to determine if the ‘rampexpyrox’ model was potentially suitable for our samples, despite the above published advice that they were not. The reviewer has now confirmed (pers. comm. 16/01/2025) that due to the low amount of carbonates in our samples that it would be worth attempting to calculate activation energies. We will therefore implement the ‘rampedpyrox’ model for analysis and presentation of the thermograms and isotope data in the revised manuscript; we thank the reviewer for this clarification.
  Regarding the normalisation of thermograms to maximum peak size, this is simply to aid visual comparison of the curves between the samples. The regressions were not based on the normalised curves, but on the volume of CO₂ evolved over the defined temperature intervals.
  As we will be implementing the ‘rampedpyrox’ model for our data analysis and presentation of the thermograms and isotope data, the figures used in the results section will be updated in the revised manuscript. Therefore, the normalised thermograms and regressions presented in the initial manuscript will likely be removed from the revised version and replaced by a discussion of the distribution of activation energies (and related ¹⁴C contents).
  Analysis and measurement detail
  
  A published paper verifying the RO methodology used in this manuscript already exists (Garnett et al., 2023) and is cited in this paper. We will add the required details such as carrier gas composition, sample masses, and flow rate to the revised manuscript. We thank the reviewer for their suggested verification checks and we will add the mass balance verifications to the revised manuscript where possible.
  The decision to not remove carbonates from our samples was taken to avoid the potential loss of carbon from labile fractions during acid treatment (Bao et al., 2019). Importantly, we note that in Hemingway et al. (2017), acid treatment of samples prior to RO resulted in a shift of 0.04 Fm ¹⁴C. This effect could shift one of our samples from having a pre-bomb ¹⁴C content to a post-bomb ¹⁴C content, or vice-versa. A similar shift in ¹⁴C content for our samples could seriously impact the interpretations in our study, and our ability to compare the ¹⁴C content of the CO₂ respired from bulk (untreated) soils in the incubation experiments (Houston et al., 2024) to the ¹⁴C content of the CO₂ evolved during the RO procedure. This is a crucial component of this work and hence why samples were not decarbonated.
  Furthermore, the detail on the treatment of these samples is stated in the manuscript, L109: “The RO sub-samples were individually dried to constant mass before milling to a fine powder to homogenise and limit potential shielding effects from aggregates. The samples were then sent to the NEIF Radiocarbon Laboratory for the RO procedure, which is described in Garnett et al. (2023)”. We acknowledge the fact that we did not acid-treat our samples is implicit rather than explicitly stated, so we will add in this detail to the revised manuscript to avoid confusing future readers. We will also add a discussion section detailing why we did not decarbonate our samples and will also report the inorganic carbon content of the bulk soil samples in the supplementary information (these are <0.5 % inorganic carbon). In this new section we will also discuss how not decarbonating our samples may have impacted our results and interpretations.
  Regarding the normalisation of ¹⁴C values, it is incorrect to state that using δ¹³C values (determined using isotope ratio mass spectrometry (IRMS) from an aliquot of the sample CO₂) to normalise ¹⁴C results represents a major break from typical procedure. Use of IRMS δ¹³C values for normalising AMS data is clearly supported in several key AMS methods papers (Donahue et al., 1990; McNichol et al., 2001) and while online AMS ¹³C measurements can be advantageous particularly for small samples (Santos et al., 2007), many labs employ IRMS values for normalisation (McIntyre et al., 2017) and quality assurance results show that the method is clearly reliable (Ascough et al., 2024).
  Data validation and verification
  
  The RO samples are sub-sets of those analysed in Houston et al. (2024), where the bulk sample %OC, %IC, pMC, and δ¹³C values are all reported (Houston et al., 2024). To aid comparison for readers, we will add these into the supplementary material in the revised manuscript. We will also add sense-checks such as isotope mass-balance calculations to the supplementary material.
  We welcome the reviewer’s point about the potential (or lack thereof) for ¹³C fractionation during the RO procedure. We will alter this section of the manuscript and discuss in greater detail the mechanisms such as different compounds combusting at different temperature ranges and the importance of organo-mineral interactions for some compound classes. However, ¹⁴C is the main focus of this manuscript (comparison of ¹⁴C content of respired CO₂ from incubation experiments and ¹⁴C content of thermal reactivity fractions).
  We will also discuss the potential for carbonate contribution as low as ~550 °C here. We note that the ¹³C values for each of the 500-650 °C RO fractions were within expected ranges for OC in these samples, but that there could be contributions from carbonate sources. As previously stated, we will be implementing the ‘rampedpyrox’ model for our data analysis and presentation of the thermograms and isotope data, so the interpretations of ¹⁴C content and temperature will be replaced in the revised manuscript by comparing ¹⁴C contents of the evolved CO₂ to activation energies.
  Concepts and terminology
  
  We will improve on our terminology usage in the revised manuscript and thank the reviewer for their suggested terms of “thermal recalcitrance” and “thermal lability” which we will use when referring to the thermal reactivity of the OC pools. We will retain the use of “bioavailable OC” when referring to the CO₂ respired from the incubation experiments. We agree with the reviewer that it is important to highlight to the reader that thermal reactivity does not have to correlate with biological turnover times. Interestingly, this was one of the motivations for undertaking this work as much previous work has been undertaken on the ‘reactivity’ of soil/sediment OC but there has been limited work comparing this to ‘bioavailable’ carbon under set environmental conditions.
  We take on board the reviewer’s comments of not using ¹⁴C ‘ages’. We had included this to provide context to the most ¹⁴C-depleted samples to highlight the potential for ¹⁴C-dead contributions but that they aren’t necessary to achieve these low ¹⁴C contents for the region. However, we will remove this interpretation in the revised manuscript to avoid inappropriate reporting of complex OC mixtures.
  Regressions, plotting, and statistics
  
  We agree with the reviewer that the regressions are not ideal as they are calculated from the mean temperature, but we now acknowledge that the ¹⁴C (or ¹³C) content could be predominantly evolved from a narrower temperature interval within the defined temperature range of CO2 collection. However, we agree with the reviewer that these plots could be clearer and welcome their data visualisation suggestions which we will implement in the revised manuscript.
  Additionally, we agree that the box and whisker plots result in a loss of crucial information about individual samples.
  The omission of the 650-800 °C RO fraction from Figure 3 was to compare the OC pools. However, we strongly disagree with the reviewer that this was a dishonest omission as the δ¹³C for the 650-800 °C RO fraction are reported in Table 2, directly below this figure.
  As we will be implementing the ‘rampedpyrox’ model for our data analysis and presentation of the thermograms and isotope data a lot of the figures used in the results section will be updated in the revised manuscript.
  We agree with the reviewer that we should remove any interpretation of statistically insignificant trends from the revised manuscript.
  
  Yours sincerely,
  Alex Houston (on behalf of all authors).
  
  Reference List
  Ascough, P., Bompard, N., Garnett, M. H., Gulliver, P., Murray, C., Newton, J.-A., and Taylor, C.: 14C MEASUREMENT OF SAMPLES FOR ENVIRONMENTAL SCIENCE APPLICATIONS AT THE NATIONAL ENVIRONMENTAL ISOTOPE FACILITY (NEIF) RADIOCARBON LABORATORY, SUERC, UK, Radiocarbon, 66, 1020–1031, https://doi.org/10.1017/RDC.2024.9, 2024.
  Bao, R., McNichol, A. P., Hemingway, J. D., Gaylord, M. C. L., and Eglinton, T. I.: Influence of Different Acid Treatments on the Radiocarbon Content Spectrum of Sedimentary Organic Matter Determined by RPO/Accelerator Mass Spectrometry, Radiocarbon, 61, 395–413, https://doi.org/10.1017/RDC.2018.125, 2019.
  Dasari, S., Garnett, M. H., and Hilton, R. G.: Leakage of old carbon dioxide from a major river system in the Canadian Arctic, PNAS Nexus, 3, pgae134, https://doi.org/10.1093/pnasnexus/pgae134, 2024.
  Dean, J. F., Billett, M. F., Turner, T. E., Garnett, M. H., Andersen, R., McKenzie, R. M., Dinsmore, K. J., Baird, A. J., Chapman, P. J., and Holden, J.: Peatland pools are tightly coupled to the contemporary carbon cycle, Global Change Biology, n/a, e16999, https://doi.org/10.1111/gcb.16999, 2023.
  Donahue, D. J., Linick, T. W., and Jull, A. J. T.: Isotope-Ratio and Background Corrections for Accelerator Mass Spectrometry Radiocarbon Measurements, Radiocarbon, 32, 135–142, https://doi.org/10.1017/S0033822200040121, 1990.
  Garnett, M. H. and Hardie, S. M. L.: Isotope (14C and 13C) analysis of deep peat CO2 using a passive sampling technique, Soil Biology and Biochemistry, 41, 2477–2483, https://doi.org/10.1016/j.soilbio.2009.09.004, 2009.
  Garnett, M. H., Hardie, S. M. L., and Murray, C.: Radiocarbon analysis reveals that vegetation facilitates the release of old methane in a temperate raised bog, Biogeochemistry, 148, 1–17, https://doi.org/10.1007/s10533-020-00638-x, 2020.
  Garnett, M. H., Pereira, R., Taylor, C., Murray, C., and Ascough, P. L.: A NEW RAMPED OXIDATION-14C ANALYSIS FACILITY AT THE NEIF RADIOCARBON LABORATORY, EAST KILBRIDE, UK, Radiocarbon, 65, 1213–1229, https://doi.org/10.1017/RDC.2023.96, 2023.
  Grant, K. E., Galy, V. V., Chadwick, O. A., and Derry, L. A.: Thermal oxidation of carbon in organic matter rich volcanic soils: insights into SOC age differentiation and mineral stabilization, Biogeochemistry, 144, 291–304, https://doi.org/10.1007/s10533-019-00586-1, 2019.
  Hemingway, J. D., Rothman, D. H., Rosengard, S. Z., and Galy, V. V.: Technical note: An inverse method to relate organic carbon reactivity to isotope composition from serial oxidation, Biogeosciences, 14, 5099–5114, https://doi.org/10.5194/bg-14-5099-2017, 2017.
  Houston, A., Garnett, M. H., and Austin, W. E. N.: Blue carbon additionality: New insights from the radiocarbon content of saltmarsh soils and their respired CO2, Limnology and Oceanography, n/a, https://doi.org/10.1002/lno.12508, 2024.
  McIntyre, C. P., Wacker, L., Haghipour, N., Blattmann, T. M., Fahrni, S., Usman, M., Eglinton, T. I., and Synal, H.-A.: Online 13C and 14C Gas Measurements by EA-IRMS–AMS at ETH Zürich, Radiocarbon, 59, 893–903, https://doi.org/10.1017/RDC.2016.68, 2017.
  McNichol, A. P., Jull, A. J. T., and Burr, G. S.: Converting AMS Data to Radiocarbon Values: Considerations and Conventions, Radiocarbon, 43, 313–320, https://doi.org/10.1017/S0033822200038169, 2001.
  Peltre, C., Fernández, J. M., Craine, J. M., and Plante, A. F.: Relationships between Biological and Thermal Indices of Soil Organic Matter Stability Differ with Soil Organic Carbon Level, Soil Science Society of America Journal, 77, 2020–2028, https://doi.org/10.2136/sssaj2013.02.0081, 2013.
  Sanderman, J. and Grandy, A. S.: Ramped thermal analysis for isolating biologically meaningful soil organic matter fractions with distinct residence times, SOIL, 6, 131–144, https://doi.org/10.5194/soil-6-131-2020, 2020.
  Santos, G. M., Moore, R. B., Southon, J. R., Griffin, S., Hinger, E., and Zhang, D.: AMS 14C Sample Preparation at the KCCAMS/UCI Facility: Status Report and Performance of Small Samples, Radiocarbon, 49, 255–269, https://doi.org/10.1017/S0033822200042181, 2007.
  Stoner, S. W., Schrumpf, M., Hoyt, A., Sierra, C. A., Doetterl, S., Galy, V., and Trumbore, S.: How well does ramped thermal oxidation quantify the age distribution of soil carbon? Assessing thermal stability of physically and chemically fractionated soil organic matter, Biogeosciences, 20, 3151–3163, https://doi.org/10.5194/bg-20-3151-2023, 2023.
  Williams, E. K., Fogel, M. L., Berhe, A. A., and Plante, A. F.: Distinct bioenergetic signatures in particulate versus mineral-associated soil organic matter, Geoderma, 330, 107–116, https://doi.org/10.1016/j.geoderma.2018.05.024, 2018.
  
  Citation: https://doi.org/10.5194/egusphere-2024-3281-AC1
RC2:
'Comment on egusphere-2024-3281', Anonymous Referee #2, 17 Mar 2025

General Comments:
This is a an important piece of work investigating the reactivity of saltmarsh soil organic carbon. The study found that 14C-depleted (older) carbon evolved from higher temperature ramped oxidation fractions, indicating that older carbon dominates the thermally recalcitrant fractions. The work does progress the field and offers new insights into our understanding of the composition of this important carbon sink. However, there are a few concerns with the manuscript that require some consideration and revision.

Specific Comments
The use of the term “pre-aged” (especially in the abstract) is ambiguous and should be clarified to the reader without needing to go back to Houston et al (2024).
In the methods section there should be a sub section on data analysis. What programmes were used, how was the data treated?
Also Line 105-106. “the deepest sample from each core being the deepest retrieved sample” – does this mean that the depth to refusal at this site is 20cm?
As regards the results section – the main query is the categorical approach to representing the data in Figure 2 and Figure 3 and using the exponential and linear regression (respectively). The categorical approach can stand alone without the regresssions, however if using regressions then the x axis should be represented as a continuous variable. Please consider revising this.
In terms of the two tables in the main body of the manuscript -is it possible to graphically represent some of this data? I think much like the graphical abstract, there is scope to conceptualize the data in diagrammatic form to increase the impact of the findings.
Is there data or any published work on the carbon accumulation rates at this site? How do the authors think the findings of this study would integrate with C org accumulation rate data.
As these samples are taken from the lower shore of the saltmarsh site can the authors speculate on how the results could vary with proximity to the terrestrial border and the greater proportion of organic carbon from autochthonous sources?
The abstract concludes with a sentence on how this study has relevance for saltmarsh management and therefore I expected a portion of the discussion to focus on this but I found this was just briefly mentioned at the end of the discussion and conclusion sections. I think greater discussion of the practical implications that the findings may have is necessary.

Citation: https://doi.org/10.5194/egusphere-2024-3281-RC2
- AC2: 'Reply on RC2', Alex Houston, 25 Mar 2025
  
  We thank Reviewer 2 for their constructive and helpful comments on our manuscript, and for their highlighting of the importance and novelty of this research:
  “This is an important piece of work investigating the reactivity of saltmarsh soil organic carbon. The study found that 14C-depleted (older) carbon evolved from higher temperature ramped oxidation fractions, indicating that older carbon dominates the thermally recalcitrant fractions. The work does progress the field and offers new insights into our understanding of the composition of this important carbon sink.”
  Below, we outline our response to each of their specific comments.
  The use of the term “pre-aged” (especially in the abstract) is ambiguous and should be clarified to the reader without needing to go back to Houston et al (2024).
  In a revised manuscript, we will change ‘pre-aged’ to ‘aged’ to clarify for the reader that the input of organic matter to blue carbon soils need not always be the same age as the in-situ production of plant material.
  In the methods section there should be a sub section on data analysis. What programmes were used, how was the data treated?
  We will add a ‘data analysis’ section to a revised manuscript. In the current version of this manuscript all data analysis and visualisation of thermograms and isotopic data was undertaken using RStudio V4.2.2. In a revised manuscript, we will use Python V3.8 to implement the ‘rampedpyrox’ package to calculate activation energy distributions for our samples from the thermograms, as also requested by Reviewer 1 (Hemingway, 2016; Hemingway et al., 2017)
  Also Line 105-106. “the deepest sample from each core being the deepest retrieved sample” – does this mean that the depth to refusal at this site is 20cm?
  No, in this study we used a golf-hole corer and its length is 20 cm. The depth to refusal at this site is >20 cm (see Miller et al., 2023), and variable across the site. In the revised manuscript we will add an explicit statement about the length of the coring device at the appropriate point in the text and this will add the necessary clarification.
  As regards the results section – the main query is the categorical approach to representing the data in Figure 2 and Figure 3 and using the exponential and linear regression (respectively). The categorical approach can stand alone without the regressions, however if using regressions then the x axis should be represented as a continuous variable. Please consider revising this.
  In the revised manuscript we will implement the ‘rampedpyrox’ package (Hemingway, 2016), based on suggestions from Reviewer 1 (we direct readers to our detailed online response letter to Reviewer 1). This package allows the calculation of continuous activation energy distributions for each ramped oxidation sample, instead of the categorical temperature binning approach we employed in the current version of the manuscript. With this new approach, we will be able to use regressions appropriately.
  In terms of the two tables in the main body of the manuscript -is it possible to graphically represent some of this data? I think much like the graphical abstract, there is scope to conceptualize the data in diagrammatic form to increase the impact of the findings.
  The data from these two tables is presented in this version of the manuscript in Figures 2 and 3. However, in the revised manuscript we will seek to improve our data visualisation to increase the impact of our findings.
  Is there data or any published work on the carbon accumulation rates at this site? How do the authors think the findings of this study would integrate with C org accumulation rate data.
  Yes, there are published carbon (C) accumulation rates for this site (Miller et al., 2023; Smeaton et al., 2024). Compared to other Scottish and UK saltmarshes, Skinflats has relatively high C accumulation rates ( Miller et al., 2023; Smeaton et al., 2024). In previously published work we showed that Skinflats accumulates carbon of a much greater ‘age’ (depleted soil ¹⁴C contents) than two other saltmarshes in Scotland (Houston et al., 2024). The combination of high carbon accumulation rates and depleted soil ¹⁴C contents implies that the Skinflats saltmarsh accumulates a high proportion of aged, most likely allochthonous C. This is potentially why we don’t see a clear decrease in the thermally labile ramped oxidation fractions with depth in this study - as much of the accumulated C has already been processed/degraded prior to deposition at the core site. Our findings in this study, that aged C has a greater thermal recalcitrance than young C, imply that sites accumulating younger C would have more thermally labile soil C. We will add detail on these interpretations and potential implications to the discussion section of the revised manuscript.
  As these samples are taken from the lower shore of the saltmarsh site can the authors speculate on how the results could vary with proximity to the terrestrial border and the greater proportion of organic carbon from autochthonous sources?
  We will add detail on this to the revised manuscript. Given our findings that more aged carbon has greater thermal recalcitrance, we anticipate that higher marsh zones, which typically have greater proportions of autochthonous C than lower marsh zones, would have a greater proportion of thermally labile C. However, it is important to recognise that young, autochthonous C can also be thermally recalcitrant. As well as with marsh zone, we expect that the proportion of C sources and thermal reactivity would vary with proximity to marsh creeks which redistribute autochthonous and allochthonous C around the site.
  Some recent work, sampling surface sediments across the saltmarsh-mudflat ecotone, has also provided preliminary evidence that low marsh zones (like in this study) have soils which store organic matter of a greater thermal reactivity than adjacent mudflat sediments (McIntosh et al., 2025).
  The abstract concludes with a sentence on how this study has relevance for saltmarsh management and therefore I expected a portion of the discussion to focus on this but I found this was just briefly mentioned at the end of the discussion and conclusion sections. I think greater discussion of the practical implications that the findings may have is necessary.
  We welcome the reviewer’s encouragement to expand on the implications of our results and will add this to the discussion section of the revised manuscript.
  
  Yours sincerely,
  Alex Houston (on behalf of all authors).
  
  Reference List
  Hemingway, J. D.: rampedpyrox: Open-source tools for thermoanalytical data analysis, 2016-, 2016.
  Hemingway, J. D., Rothman, D. H., Rosengard, S. Z., and Galy, V. V.: Technical note: An inverse method to relate organic carbon reactivity to isotope composition from serial oxidation, Biogeosciences, 14, 5099–5114, https://doi.org/10.5194/bg-14-5099-2017, 2017.
  Houston, A., Garnett, M. H., and Austin, W. E. N.: Blue carbon additionality: New insights from the radiocarbon content of saltmarsh soils and their respired CO2, Limnology and Oceanography, n/a, https://doi.org/10.1002/lno.12508, 2024.
  McIntosh, C. L., Smeaton, C., Houston, A., Chalmers, K., Carl, L., Yang, H., and Austin, W. E. N.: Soil organic carbon content and reactivity at the pioneer saltmarsh-mudflat ecotone, Copernicus Meetings, https://doi.org/10.5194/egusphere-egu25-9591, 2025.
  Miller, L. C., Smeaton, C., Yang, H., and Austin, W. E. N.: Carbon accumulation and storage across contrasting saltmarshes of Scotland, Estuarine, Coastal and Shelf Science, 282, 108223, https://doi.org/10.1016/j.ecss.2023.108223, 2023.
  Smeaton, C., Garrett, E., Koot, M. B., Ladd, C. J. T., Miller, L. C., McMahon, L., Foster, B., Barlow, N. L. M., Blake, W., Gehrels, W. R., Skov, M. W., and Austin, W. E. N.: Organic carbon accumulation in British saltmarshes, Science of The Total Environment, 926, 172104, https://doi.org/10.1016/j.scitotenv.2024.172104, 2024.
  
  Citation: https://doi.org/10.5194/egusphere-2024-3281-AC2

Alex Houston, Mark H. Garnett, Jo Smith, and William E. N. Austin

Supplement

https://doi.org/10.5194/egusphere-2024-3281-supplement

Alex Houston, Mark H. Garnett, Jo Smith, and William E. N. Austin

Viewed

Total article views: 612 (including HTML, PDF, and XML)

HTML	PDF	XML	Total	Supplement	BibTeX	EndNote
498	75	39	612	31	26	43

HTML: 498
PDF: 75
XML: 39
Total: 612
Supplement: 31
BibTeX: 26
EndNote: 43

Views and downloads (calculated since 13 Nov 2024)

Month	HTML	PDF	XML	Total
Nov 2024	136	17	8	161
Dec 2024	44	10	1	55
Jan 2025	35	6	2	43
Feb 2025	27	4	0	31
Mar 2025	53	12	2	67
Apr 2025	39	6	18	63
May 2025	21	5	3	29
Jun 2025	32	3	3	38
Jul 2025	34	9	1	44
Aug 2025	77	3	1	81

Cumulative views and downloads (calculated since 13 Nov 2024)

Month	HTML	PDF	XML	Total
Nov 2024	136	17	8	161
Dec 2024	44	10	1	55
Jan 2025	35	6	2	43
Feb 2025	27	4	0	31
Mar 2025	53	12	2	67
Apr 2025	39	6	18	63
May 2025	21	5	3	29
Jun 2025	32	3	3	38
Jul 2025	34	9	1	44
Aug 2025	77	3	1	81

Viewed (geographical distribution)

Total article views: 609 (including HTML, PDF, and XML) Thereof 609 with geography defined and 0 with unknown origin.

Country	#	Views	%

Latest update: 30 Aug 2025

Short summary

The organic carbon stored in saltmarsh soils can be up to 15,000 years old. We found that less energy is required to decompose young carbon than old carbon, i.e., young carbon tends to be more labile. We show that the labile carbon can still be up to 2,000 years old, implying that even old carbon in saltmarsh soils may contribute to greenhouse gas emissions. Protecting saltmarshes from degradation may help conserve these stores of old, labile organic carbon and hence limit CO₂ emissions.


Total:	0
HTML:	0
PDF:	0
XML:	0