The role of surface-active macromolecules in the ice nucleating ability of lignin, Snomax, and agricultural soil extracts

Abstract. Organic matter in agricultural soil dust can enhance dust's ice-nucleating ability relative to mineral dust, and thus impact local cloud formation. But how is this organic matter able to nucleate ice? We hypothesised that hydrophobic interfaces, such as the air-water interface, influence how organic matter nucleates ice, which can be quantified by measuring surface tension. Here, we investigated correlations between surfactant properties and ice-nucleating activities of amphiphilic macromolecules common in agricultural soils and known ice nucleators, namely lignin and macromolecules from Snomax. Lignin and Snomax solutions were analysed using our droplet freezing technique, FINC, and using an optical contact angle tensiometer. Results showed that lignin and Snomax solutions of increasing concentrations had increasing ice-nucleating activity and decreasing surface tension. In addition, high-speed cryo-microscopy of the same solutions revealed a preference for freezing at the air-water interface, consistent with these proxies being ice-active surfactants preferentially residing at the air-water interface, and thus hydrophobic surfaces. We then tested this relationship in field-collected agricultural soil extracts from the UK and Canada. Despite the trend observed for lignin and Snomax, there was no clear correlation between surface tension and freezing temperature of the soil extracts. This discrepancy may arise from the high complexity of the soil solutions, where hydrophobic interfaces in the bulk potentially compete with the air-water interface. Overall, we present further evidence of the role of hydrophobic interfaces in the heterogeneous ice nucleation of organic aerosols with implications for aerosol-cloud interactions.