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https://doi.org/10.5194/egusphere-2025-501
https://doi.org/10.5194/egusphere-2025-501
25 Feb 2025
 | 25 Feb 2025

Technical note: Measurements of fluorescent dissolved organic matter (FDOM) in seawater (Filter blanks, pore sizes, and storage)

Junhyeong Seo, Heejun Han, Intae Kim, and Guebuem Kim

Abstract. Fluorescent dissolved organic matter (FDOM) provides crucial information regarding the sources and characteristics of DOM in oceans. However, the FDOM measurements vary depending on filter blanks, pore sizes, and sample storage. To develop more reliable methods for FDOM measurements, in this study, we examined the uncertainties associated with different preparation methods for seawater samples. These samples identified three primary components using parallel factor analyses: terrestrial humic-like peak (C peak), marine humic-like peak (M peak), and protein-like peak (T peak). Relatively high procedural blank values were obtained from samples filtered through the pre-combusted glass fiber filter (0.7 μm pore size) and membrane filter (0.2 μm pore size) without pre-cleaning. However, the blank values became negligible when the filter was pre-washed with 5 mL of 0.1 M HCl or 20 mL of distilled water. The effects of different filter pore sizes were not observed for the C and M peak concentrations (FDOMH), but relatively low T peak values were observed for filtered samples (0.7 or 0.2 μm) relative to unfiltered samples. For all samples, FDOMH showed consistent results for 21 days (8 % ± 3 %) when stored in a refrigerator or a freezer. However, T peak concentration decreased rapidly in both filtered (15 %–50 %) and unfiltered samples (10 %–40 %) after five days, indicating considerable bacterial degradation of protein-like components within three days. Therefore, our results suggest that reliable FDOMH values can be obtained either unfiltered and filtered samples stored in either a refrigerator or freezer for three weeks, but careful sample filtration, storage, blank controls are necessary for T peak measurements.

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Journal article(s) based on this preprint

04 Sep 2025
Technical note: Measurements of fluorescent dissolved organic matter (FDOM) in seawater (filter blanks, pore sizes, and storage)
Junhyeong Seo, Heejun Han, Intae Kim, and Guebuem Kim
Biogeosciences, 22, 4423–4431, https://doi.org/10.5194/bg-22-4423-2025,https://doi.org/10.5194/bg-22-4423-2025, 2025
Short summary
Junhyeong Seo, Heejun Han, Intae Kim, and Guebuem Kim

Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on egusphere-2025-501', Philippe Massicotte, 27 Feb 2025
    • AC1: 'Reply on RC1', Junhyeong Seo, 02 Apr 2025
      • EC1: 'Reply on AC1', Huixiang Xie, 19 Apr 2025
      • AC3: 'Reply on AC1', Junhyeong Seo, 30 Apr 2025
        • AC4: 'Reply on AC3', Junhyeong Seo, 30 Apr 2025
  • RC2: 'Comment on egusphere-2025-501', Anonymous Referee #2, 08 Apr 2025

Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on egusphere-2025-501', Philippe Massicotte, 27 Feb 2025
    • AC1: 'Reply on RC1', Junhyeong Seo, 02 Apr 2025
      • EC1: 'Reply on AC1', Huixiang Xie, 19 Apr 2025
      • AC3: 'Reply on AC1', Junhyeong Seo, 30 Apr 2025
        • AC4: 'Reply on AC3', Junhyeong Seo, 30 Apr 2025
  • RC2: 'Comment on egusphere-2025-501', Anonymous Referee #2, 08 Apr 2025

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision | EF: Editorial file upload
ED: Reconsider after major revisions (07 May 2025) by Huixiang Xie
AR by Junhyeong Seo on behalf of the Authors (28 May 2025)  Author's response   Author's tracked changes   Manuscript 
ED: Referee Nomination & Report Request started (28 May 2025) by Huixiang Xie
RR by Anonymous Referee #2 (12 Jun 2025)
ED: Publish subject to minor revisions (review by editor) (13 Jun 2025) by Huixiang Xie
AR by Junhyeong Seo on behalf of the Authors (19 Jun 2025)  Author's response   Author's tracked changes   Manuscript 
ED: Publish subject to technical corrections (21 Jun 2025) by Huixiang Xie
AR by Junhyeong Seo on behalf of the Authors (23 Jun 2025)  Author's response   Manuscript 

Journal article(s) based on this preprint

04 Sep 2025
Technical note: Measurements of fluorescent dissolved organic matter (FDOM) in seawater (filter blanks, pore sizes, and storage)
Junhyeong Seo, Heejun Han, Intae Kim, and Guebuem Kim
Biogeosciences, 22, 4423–4431, https://doi.org/10.5194/bg-22-4423-2025,https://doi.org/10.5194/bg-22-4423-2025, 2025
Short summary
Junhyeong Seo, Heejun Han, Intae Kim, and Guebuem Kim
Junhyeong Seo, Heejun Han, Intae Kim, and Guebuem Kim

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Short summary
This study examines methodological uncertainties in FDOM (fluorescent dissolved organic matter) measurements due to filter blanks, pore sizes, and storage conditions. Results show that pre-cleaning filters and optimizing storage improve measurement reliability. These finding minimize procedural errors and provide clear guidelines for FDOM analysis, emphasizing the need for method standardization in marine biogeochemical research.
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