the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Soil stoichiometric characteristics and influencing factors in karst forests under micro-topography and microhabitat scales
Abstract. To quantitatively evaluate the stoichiometric characteristics of karst forest soils and their response mechanisms to complex microenvironments, the study systematically investigated soil stoichiometric traits and influencing factors across micro-topography and microhabitat scales in the Maolan karst forest. Key findings include: (1) Soil nutrients (organic carbon, total nitrogen, hydrolyzable nitrogen, available phosphorus, available potassium, total calcium, exchangeable calcium, and exchangeable magnesium) exhibited strong variability with significant spatial heterogeneity; (2) Microhabitat factors significantly influenced nutrient accumulation, though different elements showed distinct response patterns to microhabitat variations; (3) Micro-topographic parameters (slope gradient, aspect, and position) exerted indirect effects through gravity, light exposure, and erosion, driving the formation of gradient patterns in soil stoichiometry; (4) Differential response mechanisms of nutrients to abiotic factors, combined with the differential nutrient regulation and absorption strategies of various plant life forms, collectively shaped the complex stoichiometric characteristics. Synergistic interactions were observed among microhabitat-micro-topography-plant life form factors, with geomorphological abiotic factors playing predominant roles at this scale. Although biotic factors like plant life forms showed relatively weaker direct influences, their regulatory effects were closely interrelated with microhabitat-topographic factors. This multi-dimensional feedback mechanism between biotic and abiotic factors reflects the complexity of nutrient cycling in karst ecosystems.
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Status: final response (author comments only)
- RC1: 'Comment on egusphere-2025-3510', Anonymous Referee #1, 16 Sep 2025
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RC2: 'Comment on egusphere-2025-3510', Anonymous Referee #2, 03 Oct 2025
The authors studied the stoichiometric characteristics of a karst forest soil and the relationship with microtopography, microhabitat and plant life form at Maolan Nature Reserve, and found that stoichiometric characteristics are shaped by interactive effects of these three factors, especially microhabitat-micrography interactions. In general, the authors investigated complex relationships using various statistical analysis, as the effect on certain elements and stoichiometric ratios are often not uniform, it is difficult for readers to grasp the most relevant results and key messages. To improve the readability, I suggest the authors reduce the length and make descriptions on results and discussion more concise (see where to reduce in the specific comments below). In addition, more details are needed in figures or their captions so they are understandable stand alone.
Major comments
- Consistence of terms needs to be improved. A few examples
Types of microhabitat were rock surface, rock groove, and soil surface (lines 125-144), but they were coded as stone surface, stong gully and soil surface from the method (lines 182) and used in the results and discussion. I believe ‘stong’ is a typo, but it appeared eight times in the texts and sometimes is capitalized and sometimes not.
Hyphen usage: e.g. microtopography and micro-topography both appeared in the text. Microenvironment, micro-environment, etc.
Slope degrees and slop gradients: stick to one term
- Missing important information, for example
Lines 100-108: how many sites/plots were sampled and how many points were in each stratum? The authors neither report this in any tables nor figures.
Lines 145-149: The description of microhabitat showed soil layer thickness was less than 20 cm for rock surface, <20 cm for rock groove and has well-developed soil horizons for soil surface type, what was the sampling depth and how did the authors sample soils while thickness differs this much? Sampled the center of the layer with cut ring? Was the thickness of soil layer recorded? The comparability of analyzed properties and calculated stoichiometric ratios would be questionable if the sampling depths were not similar.
Specific comments
- Line 18: specify the microhabitat factors
- Lines 21-23: different instead of differential?
- Line 22: specify abiotic factors to microtopographic and microhabitat instead of using abiotic factors alone. ‘Abiotic factors’ here may mislead readers to think about temperature and other commonly used climatic factors.
- Lines 70-75: rephrase them into questions or aims?
- Line 76: what multi-scale refers to? Is not this study at the hillslope scale?
- It looks like space is often missing before each citation.
- Line 96: section 2 study area and 3 study method can be combined into one
- Lines 155-158: 0.149 mm sieve, the justification here is not convincing. Up to 2 mm is still considered as soil in commonly used soil particle size classification systems. What happened to the material not passing 0.149 mm sieve? Dropped or milled until passing the sieve? Add details about which properties were analyzed with fresh soil and which with dry soils.
- Lines 172-173: explicitly list which ratios were calculated.
- Table 1 caption, it is not a comparison, use soil properties instead of detection indicators. Is CV the coefficient variation? Add reference for which range is considered as weak, moderate and strong variability.
- Lines 191-198: section 4.1.1 might not be needed at all. The only important information I see here is the variability of all samples and only TK and TP were normally distributed. Considering the authors used soil pH to explain the pattern of exchangeable Ca in the discussion, I suggest add soil pH column to table 1.
- Section 4.1.2 Very long descriptive statistics, reduce the text to the most relevant. For example, describe the general trend, means or medians were highest on which slop degrees or increase in the order of …, which types had significant differences, and the interesting patterns to be discussed later. Similar reduction can be made throughout the sections under 4.2.
- Figure 2: Add number of points for each slope degree type either in the figure or in the caption, same applies to figures 3-6.
- Lines 229-231: wordy, can be reduced to e.g. Major soil nutrients were highest at flat land, followed by shady slopes and sunny slopes. There are many places like this can be shortened, consider using editing services or asking native speakers to improve language.
- Lines 321-325: this is because the same variable was used in correlating that ratio, it does not make sense to report this type of correlation. Report only the most relevant parts.
- I think section 4.4 does not need subsections, readers can see that one paragraph reporting results from RDA and the other from VPA.
- Figure 8: space is enough to write environmental factors in full in the biplot, or add the abbreviations to the figure caption.
- Line 336, and table 4: what type of correlation is used in correlating continuous variables and assigned ordinal variables? What is the correlation coefficient called in this case? I could not find details from the method either. It seems that results from Table 4 is not used from the results section, move to supplement instead?
- Line 359-360: plant species and plant nutrient contents appeared first time here, describe them in the method.
- Lines 360-367 and figure 9: What are stoichiometric traits and stoichiometric variation refer to? What is/are response variable(s) in VPA analysis?
- Regarding the discussion part, the authors tended to begin paragraphs by stating the importance of the topics, this fits to introduction better and completely unnecessary in the discussion. I suggest the authors focus on their findings and then put them into a larger context (findings from other studies). As an example, take a look at https://doi.org/10.5194/bg-19-2171-2022. In addition, sometimes it is not clear whether the authors referring to their results or the results from other studies in the discussion, the clarity could be improved.
- Lines 620-630: The limitation and outlook could be in a separate paragraph. No need to list detailed next steps, instead, point out the direction is sufficient.
Citation: https://doi.org/10.5194/egusphere-2025-3510-RC2
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The study investigates the drivers of stoichiometric relationships in soil properties in the Maolan karst region of southwest China. The researchers tie soil stoichiometry to microhabitats, topography, and vegetation, using multi-scale analysis of soil nutrients and plant life forms. The study provides strong ecological reasoning for observed nutrient patterns in karst systems, but there are some ways that the ms could be improved for readability and interpretability (in the figures and the text).
Specific Comments: