Preprints
https://doi.org/10.5194/egusphere-2025-2849
https://doi.org/10.5194/egusphere-2025-2849
04 Jul 2025
 | 04 Jul 2025

The Use of Newly Assimilated Photosynthates by Soil Autotrophic and Heterotrophic Respiration on a Diurnal Scale

Moeka Ono, Bhaskar Mitra, Benju Baniya, Dohee Kim, and Asko Noormets

Abstract. The regulatory role of plant carbohydrate status and root exudation on soil CO2 efflux has been demonstrated, yet the underlying mechanisms, particularly through root respiration, remain largely theoretical. In this study, we analyzed the cospectral variation of soil autotrophic (Ra) and heterotrophic (Rh) respiration components with key physiological and environmental factors, including gross primary productivity (GPP), photosynthetically active radiations (PAR), soil temperature (Ts) and volumetric water content (VWC), to evaluate their relative contributions in a subtropical mature shortleaf pine forest in the southern United States. The findings reveal a strong diurnal relationship between Rh and both GPP and PAR, in contrast to the weaker and more variable associations observed with Ra. This suggests that substrate availability was a key limitation of Rh on a diurnal basis, and that recently assimilated carbohydrates were directly discharged into the soil via root and mycorrhizal exudates. The consistent 2–4 hour time lag between Rh relative to GPP is consistent with the propagation rate of phloem pressure-concentration waves. While a diurnal peak in Rh-Ts covariance was also detected, the time lag of Rh in relation to Ts varied between positive and negative values, precluding this from being a causal relationship. Ra had a similarly strong cospectral peak with GPP as Rh, but with inconsistent lag, likely because of carbon availability from local starch reserves.

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Moeka Ono, Bhaskar Mitra, Benju Baniya, Dohee Kim, and Asko Noormets

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Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on egusphere-2025-2849', Anonymous Referee #1, 21 Jul 2025
  • RC2: 'Comment on egusphere-2025-2849', Anonymous Referee #2, 13 Aug 2025
Moeka Ono, Bhaskar Mitra, Benju Baniya, Dohee Kim, and Asko Noormets

Data sets

Software from: The Use of Newly Assimilated Photosynthates by Soil Autotrophic and Heterotrophic Respiration on a Diurnal Scale Moeka Ono https://github.com/moekaono/CRK_cont_SR/tree/main/Data

Model code and software

Software from: The Use of Newly Assimilated Photosynthates by Soil Autotrophic and Heterotrophic Respiration on a Diurnal Scale Moeka Ono https://github.com/moekaono/CRK_cont_SR/tree/main/Codes

Moeka Ono, Bhaskar Mitra, Benju Baniya, Dohee Kim, and Asko Noormets

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Short summary
Cospectral analysis was used to quantify coupling of autotrophic and heterotrophic soil respiration (Ra and Rh) to plant carbohydrate status in a subtropical pine forest in the southern US. Rh showed stronger diurnal links with gross primary productivity (GPP) than Ra, and lagged GPP by 2–4 hours, suggesting rapid transfer of carbohydrates to the soil. Soil temperature and moisture had strong cospectral peaks at synoptic scales, but were not causal drivers at the diurnal scale.
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