Preprints
https://doi.org/10.5194/egusphere-2024-3607
https://doi.org/10.5194/egusphere-2024-3607
15 Jan 2025
 | 15 Jan 2025

Carbon and Nitrogen Dynamics in Subsoils After 20 years of Added Precipitation in a Mediterranean Grassland

Leila Maria Wahab, Sora Kim, and Asmeret Asefaw Berhe

Abstract. Precipitation is a major driver of ecosystem change and physiochemical characteristics of soil. Under different climate change scenarios, increased drought frequency and changing precipitation are predicted to impact Mediterranean ecosystems, including in Northern California. Studies based on two major climate models investigated the impact of increased precipitation in parts of California where the additional precipitation occurred in winter or spring months. It was found that changing precipitation seasonality has significant impacts on plant community dynamics, microbial and fungal dynamics, and abiotic processes in soil. Subsoils are large carbon reservoirs. However, most studies investigating precipitation effects on soil organic matter (SOM) primarily focus on near-surface soils. Recent studies indicated different responses to environmental perturbation in surface (<30 cm) versus deep soils (>30 cm) due to important differences in physiochemical characteristics. Here, we present soil data at depth (~300 cm) from a 20-year precipitation manipulation experiment. We determined changes in total elemental concentration and stable isotope composition of soil C, N, δ13C, and δ15N for ambient control vs. additional precipitation in the winter and spring months. The addition of winter precipitation resulted in the largest cumulative C stock (0–300 cm), however there were no statistically significant changes in carbon stock throughout the depth profile. However, there was evidence for vertical translocation of carbon to deep soil layers, specifically of plant-derived carbon, with both winter and spring precipitation additions. The precipitation addition in winter also resulted in the highest subsoil carbon stock compared to the control (ambient) and spring treatments. Overall, added winter precipitation led to the best conditions for carbon accumulation since the added precipitation coincides with lower temperatures and improved growing conditions at our field site. This study highlights the importance of timing of precipitation events, especially with regard to deep carbon stocks (>1 m).

Publisher's note: Copernicus Publications remains neutral with regard to jurisdictional claims made in the text, published maps, institutional affiliations, or any other geographical representation in this paper. While Copernicus Publications makes every effort to include appropriate place names, the final responsibility lies with the authors. Views expressed in the text are those of the authors and do not necessarily reflect the views of the publisher.
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Journal article(s) based on this preprint

13 Aug 2025
Carbon and nitrogen dynamics in subsoils after 20 years of added precipitation in a Mediterranean grassland
Leila Maria Wahab, Sora L. Kim, and Asmeret Asefaw Berhe
Biogeosciences, 22, 3915–3930, https://doi.org/10.5194/bg-22-3915-2025,https://doi.org/10.5194/bg-22-3915-2025, 2025
Short summary
Leila Maria Wahab, Sora Kim, and Asmeret Asefaw Berhe

Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on egusphere-2024-3607', Anonymous Referee #1, 21 Jan 2025
    • AC2: 'Reply on RC1', Leila Wahab, 30 Jan 2025
    • AC4: 'Reply on RC1', Leila Wahab, 27 Feb 2025
  • AC1: 'Comment on egusphere-2024-3607', Leila Wahab, 30 Jan 2025
  • RC2: 'Comment on egusphere-2024-3607', Moritz Mainka, 07 Feb 2025
    • AC3: 'Reply on RC2', Leila Wahab, 25 Feb 2025

Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on egusphere-2024-3607', Anonymous Referee #1, 21 Jan 2025
    • AC2: 'Reply on RC1', Leila Wahab, 30 Jan 2025
    • AC4: 'Reply on RC1', Leila Wahab, 27 Feb 2025
  • AC1: 'Comment on egusphere-2024-3607', Leila Wahab, 30 Jan 2025
  • RC2: 'Comment on egusphere-2024-3607', Moritz Mainka, 07 Feb 2025
    • AC3: 'Reply on RC2', Leila Wahab, 25 Feb 2025

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision | EF: Editorial file upload
ED: Reconsider after major revisions (07 Apr 2025) by Anja Rammig
AR by Leila Wahab on behalf of the Authors (08 Apr 2025)  Author's response   Author's tracked changes   Manuscript 
ED: Referee Nomination & Report Request started (28 Apr 2025) by Anja Rammig
RR by Anonymous Referee #1 (02 May 2025)
RR by Moritz Mainka (13 May 2025)
ED: Publish subject to technical corrections (15 May 2025) by Anja Rammig
AR by Leila Wahab on behalf of the Authors (16 May 2025)  Author's response   Manuscript 

Journal article(s) based on this preprint

13 Aug 2025
Carbon and nitrogen dynamics in subsoils after 20 years of added precipitation in a Mediterranean grassland
Leila Maria Wahab, Sora L. Kim, and Asmeret Asefaw Berhe
Biogeosciences, 22, 3915–3930, https://doi.org/10.5194/bg-22-3915-2025,https://doi.org/10.5194/bg-22-3915-2025, 2025
Short summary
Leila Maria Wahab, Sora Kim, and Asmeret Asefaw Berhe
Leila Maria Wahab, Sora Kim, and Asmeret Asefaw Berhe

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Short summary
Soils are a large reservoir of carbon on land and there is uncertainty regarding how it will be affected by climate change. There is still active research about how changing precipitation patterns, a key aspect of climate change, will affect soil carbon and furthermore how vulnerable subsoils are to climate change. In this study, we studied subsoils after 20 years of experimentally manipulated precipitation shifts to see whether increasing precipitation would affect carbon amounts and chemistry.
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