Comprehensive increase in CO₂ release by drying-rewetting cycles among Japanese forests and pastureland soils and exploring predictors of increasing magnitude

Abstract. It is still difficult to precisely quantify and predict the effects of drying-rewetting cycles (DWCs) on soil carbon dioxide (CO₂) release due to the paucity of studies using constant moisture conditions equivalent to the mean water content during DWC incubation. The present study was performed to evaluate overall trends in the effects of DWCs on CO₂ release and to explore environmental and soil predictors for variations in the effect size in 10 Japanese forests and pastureland soils variously affected by volcanic ash during their pedogenesis. Over an 84-day incubation period including three DWCs, CO₂ release was 1.3- to 3.7-fold greater than under continuous constant moisture conditions (p < 0.05) with the same mean water content as in the DWC incubations. Analysis of the relations between this increasing magnitude of CO₂ release by DWCs (IF_CO2) and various environmental and soil properties revealed significant positive correlations between IF_CO2 and soil organometal complex contents (p < 0.05), especially pyrophosphate extractable aluminum (Alp) content (r = 0.74). Molar ratios of soil total carbon (C) and pyrophosphate-extractable C (Cp) to Alp contents and soil carbon content-specific CO₂ release rate under continuous constant moisture conditions (qCO₂_soc) were also correlated with IF_CO2 (p < 0.05). The covariations among Alp, total C, and Cp to Alp molar ratios and qCO₂_soc suggested Alp as the primary predictor of IF_CO2. Whereas soil microbial biomass C and nitrogen (N) levels were significantly lower in DWCs than under continuous constant moisture conditions, there was no significant relation between the microbial biomass decrease and IF_CO2. The present study showed a comprehensive increase in soil CO₂ release by DWC in Japanese forests and pastureland soils, suggesting that Alp is a predictor of the effect size likely due to vulnerability of organo-Al complexes to DWC.