the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
| 08 May 2023
Evidence of cryptic methane cycling and non-methanogenic methylamine consumption in the sulfate-reducing zone of sediment in the Santa Barbara Basin, California
Abstract. The recently discovered cryptic methane cycle in the sulfate-reducing zone of marine and wetland sediments couples methylotrophic methanogenesis to anaerobic oxidation of methane (AOM). Here we present evidence of cryptic methane cycling activity within the upper regions of the sulfate-reducing zone, along a depth transect within the Santa Barbara Basin, off the coast of California, USA. The top 0–20 cm of sediment from each station was subjected to geochemical analyses and radiotracer incubations using 35S-SO42-, 14C-mono-methylamine, and 14C- CH4 to find evidence of cryptic methane cycling. Methane concentrations were consistently low (~3 to ~16 µM) across the depth transect, despite AOM rates increasing with decreasing water depth (from max 0.05 nmol cm-3 d-1 at the deepest station to max 1.8 nmol cm-3 d-1 at the shallowest station). Porewater sulfate concentrations remained high (~23 mM to ~29 mM), despite the detection of sulfate reduction activity from 35S-SO42- incubations with rates up to 134 nmol cm-3 d-1. Metabolomic analysis showed that substrates for methanogenesis (i.e., acetate, methanol and methylamines) were mostly below the detection limit in the porewater, but some samples from the 1–2 cm depth section showed non-quantifiable evidence of these substrates, indicating their rapid turnover. Estimated methanogenesis from mono-methylamine ranged from 0.2 nmol to 0.5 nmol cm-3 d-1. Discrepancies between the rate constants (K1) of methanogenesis (from 14C- mono-methylamine) and AOM (from either 14C- mono-methylamine-derived 14C-CH4 or from directly injected 14C-CH4) suggest the activity of a separate, concurrent metabolic process directly metabolizing mono-methylamine to inorganic carbon. We conclude that the results presented in this work show strong evidence of cryptic methane cycling occurring within the top 20 cm of sediment in the Santa Barbara Basin. The rapid cycling of carbon between methanogenesis and methanotropy likely prevents major build-up of methane in the sulfate-reducing zone. Furthermore, our data suggest that methylamine is utilized by both methanogenic archaea capable of methylotrophic methanogenesis and non-methanogenic microbial groups. We hypothesize that sulfate reduction is responsible for the additional methylamine turnover but further investigation is needed to elucidate this metabolic activity.
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Status: closed
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RC1: 'Comment on egusphere-2023-909', Anonymous Referee #1, 14 May 2023
Review
This study “Evidence of cryptic methane cycling and non-methanogenic methylamine consumption in the sulfate-reducing zone of sediment in the Santa Barbara Basin, California” presents evidence of cryptic methane cycling activity within the upper regions of the sulfate-reducing zone, along a depth transect within the Santa Barbara Basin by the combination of geochemical analysis, metabolic analysis and radio-tracer labelling.The topic of cryptic methane cycling is of high interest to this field, the data is of good quality and the manuscript is well written. I find the topic of this study certainly interesting and suitable for this journal, and there are only a few minor points which preclude its publication at this moment.
Minor
Line 119 make
Line 148 stations’
Line 189 7 to seven
Line 223 would not call an NMR based method for porewater concentrations of acetate, methanol.. a metabolomic analysis. Rather It usually means analyzing proteins.
Line 289 Eq. 7
Line 319 What’s the point of defining a seemingly strange rate constant here? Any reference?
Line 430-432 what does this mean exactly
Line 508-510 It is not a suitable or should not be called a hypothesis in discussion part. Moreover, the hypothesis is not tested with strong proof either from literature or this study.
Citation: https://doi.org/10.5194/egusphere-2023-909-RC1 -
AC1: 'Reply on RC1', Sebastian Krause, 17 Aug 2023
Referee Comments
Author Response
Referee 1-Review
RC1 Comment: This studyEvidence of cryptic methane cycling and non-methanogenic methylamine consumption in the sulfate-reducing zone of sediment in the Santa Barbara Basin, California"presents evidence of cryptic methane cycling activity within the upper regions of the sulfate-reducing zone, along a depth transect within the Santa Barbara Basin by the combination of geochemical analysis, metabolic analysis and radio-tracer labelling. The topic of cryptic methane cycling is of high interest to this field, the data is of good quality and the manuscript is well written. I find the topic of this study certainly interesting and suitable for this journal, and there are only a few minor points which preclude its publication at this moment.
Authors' Response: We would like to thank the referee for the positive critique on the present manuscript. Below we address the comments and edits from the referee.
Minor
RC1 Comment: Line 119 make
Authors' Response: Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 148 stations’
Authors' Response:Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 189 7 to seven
Authors' Response: Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 223 would not call an NMR based method for porewater concentrations of acetate, methanol.. a metabolomic analysis. Rather It usually means analyzing proteins.
Authors' Response: Thanks to the referee for the comment. We are of course focused on the mixtures of small organics (from pore water directly (centrifuged) not dried down) that can be identified by performing spectral library matching (screening over 1000 potential metabolites) in spectra that were collected - in the same way as these spectra. Acetate, methanol etc. at their natural concentrations. Efforts to "dry down" samples and look for a longer list of non-volatile organics (amino acids, sugars etc.) is possible but not done here as the focus for this study is on the fermentation products, etc., which could be altered by drying down process (lyophilization or speed -vac etc.). The spectral optimization is for the small molecular weight molecules (generally less than 300 amu) - highly mobile (and highly resolved) peaks - so not the proteins in this case (broader lines and at natural concentrations not high enough to produce significant contributions to any of the spectra). Further, quantification of the small metabolites reported (acetate, methanol, etc.) is accomplish by incorporation of a known quantity of an internal standard (NIST certified) in to the sample that matches the collection conditions of the largely commercially developed library (Cheneaux).
However, we do see the referee’s point on clarity of the title. Since the present manuscript is focused on methylotrophic methanogenesis and thus interested in the presence and availability of methanogenic substrates we have changed the chapter title to be more in line with the focus of the paper. We plan to change the title to read: "Determination of methanogenic substrates in porewater"
RC1 Comment: Line 289 Eq. 7
Authors' Response: Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 319 What’s the point of defining a seemingly strange rate constant here? Any reference?
Authors' Response: Thank you for the comment. We define the rate constant k in section 2.5.4 because they are plotted in Figure 1 to show how they compare. More importantly the rate constants provide evidence that 14C-mono-methylamine is consumed by a non-methanogenic process and inflate the ex-situ rates for AOM-MMA. We discuss this in detail in discussion sections 4.2 and 4.3. We plan to add information at the beginning of the method section to clarify the purpose of the constant calculations.
RC1 Comment: Line 430-432 what does this mean exactly
Authors' Response: Thank you to the referee for the comment. We realize this sentence was not written clearly. We plan to rewrite the sentence in the new version of the manuscript as follows: “This pattern suggests that the partial pressure of methane was likely at thermodynamic equilibrium between methanogenesis and AOM (compare, e.g., with Conrad 1999)."
RC1 Comment: Line 508-510 It is not a suitable or should not be called a hypothesis in discussion part. Moreover, the hypothesis is not tested with strong proof either from literature or this study.
Authors' Response: Thank you for the comment we plan to change the phrasing accordingly in the new version:
Citation: https://doi.org/10.5194/egusphere-2023-909-AC1
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AC1: 'Reply on RC1', Sebastian Krause, 17 Aug 2023
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RC2: 'Comment on egusphere-2023-909', Anonymous Referee #2, 19 Jul 2023
The manuscript by Krause et al. presents compelling evidence for cryptic methane cycling and methylamine consumption in sediments from Santa Barbara Basin (SBB). This is a very well-designed study and the results are presented in a straightforward way. I have absolutely nothing to criticize on the methods, except perhaps that the radiotracer rate measurements were not performed in triplicates. Such measurements usually have a notoriously high variability, even on a very small spatial scale. In most
Therefore, I am wondering why to replicates were taken. There might not have been more cores available, but at least this should be mentioned somewhere.
Minor comments:
line 97: what do you mean by “myriad environments”? This phrase was already marked yellow in the pdf, perhaps someone else was questioning it already?
line 107: remove “strong”, any oxygen fluctuation can cause these conditions
line 122: remove “the” before coastal marine environments
line 126: …within OMZ’s is virtually unknown
line 180: Isn’t this company called “Scott Specialty Gases” since a few years? I know the old term Scotty, but I think that has changed since they were taken over by another company
line 196-206: That part should be restructured. At first I was at loss why there are suddenly 26 ml serum bottles, but then in line 203 you mention that they are used for the water from the syringes. It would make it easier for the readers if you could mention the use of the serum bottles in the first sentence and then describe how they are prepared and how the water is transferred.
line 252: As mentioned before, why did you take only three mini cores for the three processes. i.e no replicates?
line 336: add a komma after “trends”
line 363f: Why are the SRR for the top 5 cm missing? You only state that they are, which is visible on the figure, but an explanation would be appreciated.
lines 433 and 436: In one sentence you write that the methane concentrations are “relatively constant”, then a bit later you state that there are “non-linear” methane trends. That is somewhat confusing.
line 440: I suggest rephrasing the start of the sentence to “As there is considerable methanogenic activity even at the……”
line 458: Remove “porewater”, it is already mentioned before
line 459: Could you convert the concentration to µM, so it would be easier to compare to the value mentioned before?
line 489: compared to AOM-MMA
line 494-494: Why is that? This sentence is a statement, but I don’t see an explanation for it.
line 500f. This sentence is a bit hard to follow. I would suggest splitting it: “Methylamines are the simplest alkylated amines. They are derived from the degradation of choline and betaine and found in plant and phytoplankton biomass (citation).”
line 549 and 555: The pathway is called “Wood-Ljungdahl”
line 559: Replace “But” by “However, “
Citation: https://doi.org/10.5194/egusphere-2023-909-RC2 -
AC2: 'Reply on RC2', Sebastian Krause, 17 Aug 2023
RC2 Comment: The manuscript by Krause et al. presents compelling evidence for cryptic methane cycling and methylamine consumption in sediments from Santa Barbara Basin (SBB). This is a very well-designed study and the results are presented in a straightforward way. I have absolutely nothing to criticize on the methods, except perhaps that the radiotracer rate measurements were not performed in triplicates. Such measurements usually have a notoriously high variability, even on a very small spatial scale. In most
Authors' Response: We would like to thank the referee for reading the manuscript and providing positive critiques. Below we address all the referee’s comments and/or edits of the present manuscript.
RC2 Comment: Therefore, I am wondering why no replicates were taken. There might not have been more cores available, but at least this should be mentioned somewhere.
Authors' Response: Thank you for the comment. The referee is of course right that spatial heterogeneity contributes to variability in the ex-situ rates that we are interested in. The reason we did not do replicate radiotracer experiments is in fact because not enough sediment cores were available to perform replicate incubations. We plan to add a short sentence at the end of section 2.5 of the new version to clarify this.
Minor comments:
RC2 Comment: line 97: what do you mean by “myriad environments”? This phrase was already marked yellow in the pdf, perhaps someone else was questioning it already?
Authors' Response: Thank you, we plan to remove the word “myriad” from the sentence and reword the sentence to be clearer in the new version.
RC2 Comment: line 107: remove “strong”, any oxygen fluctuation can cause these conditions
Authors' Response: Thank you, we agree. We plan to remove the word “strong” in the new version of the manuscript.
RC2 Comment: line 122: remove “the” before coastal marine environments
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 126: …within OMZ’s is virtually unknown
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 180: Isn’t this company called “Scott Specialty Gases” since a few years? I know the old term Scotty, but I think that has changed since they were taken over by another company
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 196-206: That part should be restructured. At first I was at loss why there are suddenly 26 ml serum bottles, but then in line 203 you mention that they are used for the water from the syringes. It would make it easier for the readers if you could mention the use of the serum bottles in the first sentence and then describe how they are prepared and how the water is transferred.
Authors' Response: Thank you for pointing this out. We plan to restructure the paragraph to be clearer in the new version of the manuscript.
RC2 Comment: line 252: As mentioned before, why did you take only three mini cores for the three processes. i.e no replicates?
Authors' Response: Thank you for the comment. As said previously, the referee is of course right that spatial heterogeneity contributes to variability in the ex-situ rates that we are interested in. The reason we did not do replicate radiotracer experiments is in fact because not enough sediment cores were available to perform replicate incubations. We plan to add a short sentence at the end of section 2.5 of the new version to clarify this.
RC2 Comment: line 336: add a komma after “trends”
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 363f: Why are the SRR for the top 5 cm missing? You only state that they are, which is visible on the figure, but an explanation would be appreciated.
Authors' Response: SRR data are missing in the top 5 cm at SDRO is due to post-cruise analytical issues. We plan to rewrite the sentence in the new version to clarify the reason for the missing SRR data.
RC2 Comment: lines 433 and 436: In one sentence you write that the methane concentrations are “relatively constant”, then a bit later you state that there are “non-linear” methane trends. That is somewhat confusing.
Authors' Response: Thank you, we plan to reword the sentence for better consistency in the wording.
RC2 Comment: line 440: I suggest rephrasing the start of the sentence to “As there is considerable methanogenic activity even at the……”
Authors' Response: Thank you for the suggestion, we plan to adopt the suggestion into the new version of the manuscript.
RC2 Comment: line 458: Remove “porewater”, it is already mentioned before
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 459: Could you convert the concentration to µM, so it would be easier to compare to the value mentioned before?
Authors' Response: Thank you, we will convert the concentration to µM for better comparison in the new version.
RC2 Comment: line 489: compared to AOM-MMA
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 494-494: Why is that? This sentence is a statement, but I don’t see an explanation for it.
Authors' Response: The interpretation of the statement is actually in the following sentence.
RC2 Comment: line 500f. This sentence is a bit hard to follow. I would suggest splitting it: “Methylamines are the simplest alkylated amines. They are derived from the degradation of choline and betaine and found in plant and phytoplankton biomass (citation).”
Authors' Response: Thank you for the suggestion, we plan to adopt it in the new version.
RC2 Comment: line 549 and 555: The pathway is called “Wood-Ljungdahl”
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 559: Replace “But” by “However, “
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
Citation: https://doi.org/10.5194/egusphere-2023-909-AC2
-
AC2: 'Reply on RC2', Sebastian Krause, 17 Aug 2023
Interactive discussion
Status: closed
-
RC1: 'Comment on egusphere-2023-909', Anonymous Referee #1, 14 May 2023
Review
This study “Evidence of cryptic methane cycling and non-methanogenic methylamine consumption in the sulfate-reducing zone of sediment in the Santa Barbara Basin, California” presents evidence of cryptic methane cycling activity within the upper regions of the sulfate-reducing zone, along a depth transect within the Santa Barbara Basin by the combination of geochemical analysis, metabolic analysis and radio-tracer labelling.The topic of cryptic methane cycling is of high interest to this field, the data is of good quality and the manuscript is well written. I find the topic of this study certainly interesting and suitable for this journal, and there are only a few minor points which preclude its publication at this moment.
Minor
Line 119 make
Line 148 stations’
Line 189 7 to seven
Line 223 would not call an NMR based method for porewater concentrations of acetate, methanol.. a metabolomic analysis. Rather It usually means analyzing proteins.
Line 289 Eq. 7
Line 319 What’s the point of defining a seemingly strange rate constant here? Any reference?
Line 430-432 what does this mean exactly
Line 508-510 It is not a suitable or should not be called a hypothesis in discussion part. Moreover, the hypothesis is not tested with strong proof either from literature or this study.
Citation: https://doi.org/10.5194/egusphere-2023-909-RC1 -
AC1: 'Reply on RC1', Sebastian Krause, 17 Aug 2023
Referee Comments
Author Response
Referee 1-Review
RC1 Comment: This studyEvidence of cryptic methane cycling and non-methanogenic methylamine consumption in the sulfate-reducing zone of sediment in the Santa Barbara Basin, California"presents evidence of cryptic methane cycling activity within the upper regions of the sulfate-reducing zone, along a depth transect within the Santa Barbara Basin by the combination of geochemical analysis, metabolic analysis and radio-tracer labelling. The topic of cryptic methane cycling is of high interest to this field, the data is of good quality and the manuscript is well written. I find the topic of this study certainly interesting and suitable for this journal, and there are only a few minor points which preclude its publication at this moment.
Authors' Response: We would like to thank the referee for the positive critique on the present manuscript. Below we address the comments and edits from the referee.
Minor
RC1 Comment: Line 119 make
Authors' Response: Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 148 stations’
Authors' Response:Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 189 7 to seven
Authors' Response: Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 223 would not call an NMR based method for porewater concentrations of acetate, methanol.. a metabolomic analysis. Rather It usually means analyzing proteins.
Authors' Response: Thanks to the referee for the comment. We are of course focused on the mixtures of small organics (from pore water directly (centrifuged) not dried down) that can be identified by performing spectral library matching (screening over 1000 potential metabolites) in spectra that were collected - in the same way as these spectra. Acetate, methanol etc. at their natural concentrations. Efforts to "dry down" samples and look for a longer list of non-volatile organics (amino acids, sugars etc.) is possible but not done here as the focus for this study is on the fermentation products, etc., which could be altered by drying down process (lyophilization or speed -vac etc.). The spectral optimization is for the small molecular weight molecules (generally less than 300 amu) - highly mobile (and highly resolved) peaks - so not the proteins in this case (broader lines and at natural concentrations not high enough to produce significant contributions to any of the spectra). Further, quantification of the small metabolites reported (acetate, methanol, etc.) is accomplish by incorporation of a known quantity of an internal standard (NIST certified) in to the sample that matches the collection conditions of the largely commercially developed library (Cheneaux).
However, we do see the referee’s point on clarity of the title. Since the present manuscript is focused on methylotrophic methanogenesis and thus interested in the presence and availability of methanogenic substrates we have changed the chapter title to be more in line with the focus of the paper. We plan to change the title to read: "Determination of methanogenic substrates in porewater"
RC1 Comment: Line 289 Eq. 7
Authors' Response: Thank you for catching the typo. We plan to correct this in the new version of the manuscript.
RC1 Comment: Line 319 What’s the point of defining a seemingly strange rate constant here? Any reference?
Authors' Response: Thank you for the comment. We define the rate constant k in section 2.5.4 because they are plotted in Figure 1 to show how they compare. More importantly the rate constants provide evidence that 14C-mono-methylamine is consumed by a non-methanogenic process and inflate the ex-situ rates for AOM-MMA. We discuss this in detail in discussion sections 4.2 and 4.3. We plan to add information at the beginning of the method section to clarify the purpose of the constant calculations.
RC1 Comment: Line 430-432 what does this mean exactly
Authors' Response: Thank you to the referee for the comment. We realize this sentence was not written clearly. We plan to rewrite the sentence in the new version of the manuscript as follows: “This pattern suggests that the partial pressure of methane was likely at thermodynamic equilibrium between methanogenesis and AOM (compare, e.g., with Conrad 1999)."
RC1 Comment: Line 508-510 It is not a suitable or should not be called a hypothesis in discussion part. Moreover, the hypothesis is not tested with strong proof either from literature or this study.
Authors' Response: Thank you for the comment we plan to change the phrasing accordingly in the new version:
Citation: https://doi.org/10.5194/egusphere-2023-909-AC1
-
AC1: 'Reply on RC1', Sebastian Krause, 17 Aug 2023
-
RC2: 'Comment on egusphere-2023-909', Anonymous Referee #2, 19 Jul 2023
The manuscript by Krause et al. presents compelling evidence for cryptic methane cycling and methylamine consumption in sediments from Santa Barbara Basin (SBB). This is a very well-designed study and the results are presented in a straightforward way. I have absolutely nothing to criticize on the methods, except perhaps that the radiotracer rate measurements were not performed in triplicates. Such measurements usually have a notoriously high variability, even on a very small spatial scale. In most
Therefore, I am wondering why to replicates were taken. There might not have been more cores available, but at least this should be mentioned somewhere.
Minor comments:
line 97: what do you mean by “myriad environments”? This phrase was already marked yellow in the pdf, perhaps someone else was questioning it already?
line 107: remove “strong”, any oxygen fluctuation can cause these conditions
line 122: remove “the” before coastal marine environments
line 126: …within OMZ’s is virtually unknown
line 180: Isn’t this company called “Scott Specialty Gases” since a few years? I know the old term Scotty, but I think that has changed since they were taken over by another company
line 196-206: That part should be restructured. At first I was at loss why there are suddenly 26 ml serum bottles, but then in line 203 you mention that they are used for the water from the syringes. It would make it easier for the readers if you could mention the use of the serum bottles in the first sentence and then describe how they are prepared and how the water is transferred.
line 252: As mentioned before, why did you take only three mini cores for the three processes. i.e no replicates?
line 336: add a komma after “trends”
line 363f: Why are the SRR for the top 5 cm missing? You only state that they are, which is visible on the figure, but an explanation would be appreciated.
lines 433 and 436: In one sentence you write that the methane concentrations are “relatively constant”, then a bit later you state that there are “non-linear” methane trends. That is somewhat confusing.
line 440: I suggest rephrasing the start of the sentence to “As there is considerable methanogenic activity even at the……”
line 458: Remove “porewater”, it is already mentioned before
line 459: Could you convert the concentration to µM, so it would be easier to compare to the value mentioned before?
line 489: compared to AOM-MMA
line 494-494: Why is that? This sentence is a statement, but I don’t see an explanation for it.
line 500f. This sentence is a bit hard to follow. I would suggest splitting it: “Methylamines are the simplest alkylated amines. They are derived from the degradation of choline and betaine and found in plant and phytoplankton biomass (citation).”
line 549 and 555: The pathway is called “Wood-Ljungdahl”
line 559: Replace “But” by “However, “
Citation: https://doi.org/10.5194/egusphere-2023-909-RC2 -
AC2: 'Reply on RC2', Sebastian Krause, 17 Aug 2023
RC2 Comment: The manuscript by Krause et al. presents compelling evidence for cryptic methane cycling and methylamine consumption in sediments from Santa Barbara Basin (SBB). This is a very well-designed study and the results are presented in a straightforward way. I have absolutely nothing to criticize on the methods, except perhaps that the radiotracer rate measurements were not performed in triplicates. Such measurements usually have a notoriously high variability, even on a very small spatial scale. In most
Authors' Response: We would like to thank the referee for reading the manuscript and providing positive critiques. Below we address all the referee’s comments and/or edits of the present manuscript.
RC2 Comment: Therefore, I am wondering why no replicates were taken. There might not have been more cores available, but at least this should be mentioned somewhere.
Authors' Response: Thank you for the comment. The referee is of course right that spatial heterogeneity contributes to variability in the ex-situ rates that we are interested in. The reason we did not do replicate radiotracer experiments is in fact because not enough sediment cores were available to perform replicate incubations. We plan to add a short sentence at the end of section 2.5 of the new version to clarify this.
Minor comments:
RC2 Comment: line 97: what do you mean by “myriad environments”? This phrase was already marked yellow in the pdf, perhaps someone else was questioning it already?
Authors' Response: Thank you, we plan to remove the word “myriad” from the sentence and reword the sentence to be clearer in the new version.
RC2 Comment: line 107: remove “strong”, any oxygen fluctuation can cause these conditions
Authors' Response: Thank you, we agree. We plan to remove the word “strong” in the new version of the manuscript.
RC2 Comment: line 122: remove “the” before coastal marine environments
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 126: …within OMZ’s is virtually unknown
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 180: Isn’t this company called “Scott Specialty Gases” since a few years? I know the old term Scotty, but I think that has changed since they were taken over by another company
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 196-206: That part should be restructured. At first I was at loss why there are suddenly 26 ml serum bottles, but then in line 203 you mention that they are used for the water from the syringes. It would make it easier for the readers if you could mention the use of the serum bottles in the first sentence and then describe how they are prepared and how the water is transferred.
Authors' Response: Thank you for pointing this out. We plan to restructure the paragraph to be clearer in the new version of the manuscript.
RC2 Comment: line 252: As mentioned before, why did you take only three mini cores for the three processes. i.e no replicates?
Authors' Response: Thank you for the comment. As said previously, the referee is of course right that spatial heterogeneity contributes to variability in the ex-situ rates that we are interested in. The reason we did not do replicate radiotracer experiments is in fact because not enough sediment cores were available to perform replicate incubations. We plan to add a short sentence at the end of section 2.5 of the new version to clarify this.
RC2 Comment: line 336: add a komma after “trends”
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 363f: Why are the SRR for the top 5 cm missing? You only state that they are, which is visible on the figure, but an explanation would be appreciated.
Authors' Response: SRR data are missing in the top 5 cm at SDRO is due to post-cruise analytical issues. We plan to rewrite the sentence in the new version to clarify the reason for the missing SRR data.
RC2 Comment: lines 433 and 436: In one sentence you write that the methane concentrations are “relatively constant”, then a bit later you state that there are “non-linear” methane trends. That is somewhat confusing.
Authors' Response: Thank you, we plan to reword the sentence for better consistency in the wording.
RC2 Comment: line 440: I suggest rephrasing the start of the sentence to “As there is considerable methanogenic activity even at the……”
Authors' Response: Thank you for the suggestion, we plan to adopt the suggestion into the new version of the manuscript.
RC2 Comment: line 458: Remove “porewater”, it is already mentioned before
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 459: Could you convert the concentration to µM, so it would be easier to compare to the value mentioned before?
Authors' Response: Thank you, we will convert the concentration to µM for better comparison in the new version.
RC2 Comment: line 489: compared to AOM-MMA
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 494-494: Why is that? This sentence is a statement, but I don’t see an explanation for it.
Authors' Response: The interpretation of the statement is actually in the following sentence.
RC2 Comment: line 500f. This sentence is a bit hard to follow. I would suggest splitting it: “Methylamines are the simplest alkylated amines. They are derived from the degradation of choline and betaine and found in plant and phytoplankton biomass (citation).”
Authors' Response: Thank you for the suggestion, we plan to adopt it in the new version.
RC2 Comment: line 549 and 555: The pathway is called “Wood-Ljungdahl”
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
RC2 Comment: line 559: Replace “But” by “However, “
Authors' Response: Thank you. We plan to correct this in the new version of the manuscript.
Citation: https://doi.org/10.5194/egusphere-2023-909-AC2
-
AC2: 'Reply on RC2', Sebastian Krause, 17 Aug 2023
Peer review completion
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Cited
1 citations as recorded by crossref.
Sebastian Jian Ernst Krause
Jiarui Liu
David J. Yousavich
DeMarcus Robinson
David Hoyt
Qianhui Qin
Frank Wenzhoefer
Felix Janssen
David Valentine
The requested preprint has a corresponding peer-reviewed final revised paper. You are encouraged to refer to the final revised version.
- Preprint
(1723 KB) - Metadata XML
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Supplement
(1066 KB) - BibTeX
- EndNote
- Final revised paper