Mountain ecosystems present a complex challenge in disentangling the drivers of their long-term transformation. The subalpine belt of the Pyrenees, once forested, is now characterised by a mosaic of forest patches and open landscapes, and represents a key region in herding practices. However, the relative contributions of climate and human activity in driving its change remain intensely contested. We present a high-resolution, multi-proxy reconstruction of landscape dynamics over the last 25,000 years from the southern Pyrenees. By integrating mammal herbivores, fire regime and vegetation dynamics through sedimentary ancient DNA, pollen and charcoal analyses we aim to compare these proxies and identify the timing and drivers of vegetation change and to distinguish anthropogenic from climatic forces. Our results reveal a multi-phase evolution, with Lateglacial and postglacial forest expansion followed by Mid-Holocene openness, initially triggered by Neoglacial cooling but subsequently maintained by pastoral activities. We find that humans opportunistically exploited this openness from the Mid-Holocene onwards, later transitioning to active landscape management through fires and grazing. This study demonstrates that current Pyrenean landscapes are not a product of either humans or climate, but of their synergy over millennia. Effective conservation strategies must integrate an understanding of this deep ecological history with the emulation of traditional practices to preserve these unique, human-shaped biodiversity hotspots.