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# Supplement to: Gabriel Fénisse*, Manuel Chevalier, Odile Peyron, David Vincent Bekaert, Pierre-Henri Blard: Advancing Last Glacial Maximum paleoclimate reconstructions in Europe using pollen data: a multi-method (mega)biomization approach, EGUsphere [preprint], https://doi.org/10.5194/egusphere-2026-1590, 2026. #
# Contact: *Gabriel Fénisse (gabriel.fenisse@univ-lorraine.fr)[Centre de Recherches Pétrographiques et Géochimiques, Vandoeuvre-les-Nancy, 2025] #
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List of Supplementary material

1. **"A-SynthesisFile"** – Compilation of pollen sequences used in this study (`Dataset_PollenCooling.xlsx`).

2. **"B-ModernCalibrationDatasets"** – Modern calibration dataset including climate variables and pollen abundances (`CalibrationDataset_EMPD2.xlsx`).
   **"DatasetFoss_Int"** – Initial fossil pollen sequences from La Grande Pile (de Beaulieu & Reille, 1989; de Beaulieu, 1992; de Beaulieu & Reille, 1992) and Lake Bouchet (de Beaulieu et al., 1990), corresponding respectively to `GPILXX_age_model_pollen_count.tab` and `Lac_du_Bouchet_age_model_pollen_count.tab`.

3. **"C-DatasetFoss_Int"** – Original fossil pollen datasets used in this study, including pollen abundances and harmonized taxonomic labels:
   * **"LDB"**: Lake Bouchet
   * **"LGP"**: La Grande Pile
   * **"Table1"**: datasets associated with the results presented in Table 1.

4. **"D-AgeModel"** – Predicted chronologies for the sequences from La Grande Pile and Lake Bouchet (`ModeleAge_LGP.txt`, `ModeleAge_LGP2.txt`, and `ModeleAge_LDB.txt`).

5. **"E-HarmonizationTable"** – (Mega)biome harmonization tables used in this study (`Harmonization_table.txt`) from modern and fossil states and comparison of taxon names before and after harmonization (`Detail-HarmonizationTable_Results-Biome.xlsx` and `Detail-HarmonizationTable_Results-Megabiome.xlsx`).

6. **"F-Tools_BiomeScheme"** – Biomization tools including:
   * (i) the Taxon-to-PFT matrix (`Taxpftpi_Class1.csv`)
   * (ii) the PFT-to-Biome matrix (`biopftpi_Class1.csv`).

7. **"G-Tools_MegabiomeScheme"** – Megabiomization tools including:
   * (i) pollen abundance weighting used for PFT score calculation (`pollen_variable.csv`)
   * (ii) matrices linking taxa to PFTs and PFTs to megabiomes following Li et al. (2025) (`Taxon_PFT_Europe.csv` and `PFT_Megabiomes_Europe.csv`).

8. **"H-(Mega)biomisationProcess"** – Pollen-based (mega)biomization algorithm in R, tools and input datasets used for biomization (“Biome”) and megabiomization (“Megabiome”) procedures.

9. **"I-LocalCalibration_Results"** – (Mega)biomization algorithm results at Lake Bouchet and fossil sites from Table 1.

10. **"J-ReconstructionMethods"** – R scripts for pollen-based climate reconstructions using:
   * MAT with biomization (`MAT-biomization.R`)
   * WA-PLS with biomization (`WA-PLS-biomization.R`)
   * CREST with biomization (`CREST-biomization.R`).
**"FormatageBiome"** computes final reconstructions from (mega)biome scores.

11. **"K-TANNGlobalCalibration_Results"** – TANN reconstruction results from global calibration datasets using the three pollen-based reconstruction methods (`GlobalCalibration_MAT.csv`, `GlobalCalibration_WA-PLS.csv`, and `GlobalCalibration_CREST.csv`).

12. **"L-LOOCV_Codes"** – R scripts used to evaluate pollen-based climate reconstructions through leave-one-out cross-validation (LOOCV):
* MAT with (mega)biomization (`LOOCV_MAT.R`)
* WA-PLS (`LOOCV_WAPLS.R`)
* CREST (`LOOCV_CREST.R`).

