<p>Four adjacent lakes (Arco, Budd, Deming, and Josephine) within Itasca State Park in Minnesota, USA are reported to be meromictic in the scientific literature. However, seasonally persistent chemoclines have never been documented. We collected seasonal profiles of temperature and specific conductance and placed temperature sensor chains in two lakes for ~ 1 year to explore whether these lakes remain stratified through seasonal mixing events, and what factors contribute to their stability. The results indicate that all lakes are predominantly thermally stratified and are prone to mixing in isothermal periods during spring and fall. Despite brief, semi-annual erosion of thermal stratification, Deming Lake showed no signs of complete mixing from 2006 to 2009 and 2019&ndash;2022 and is likely meromictic. Geochemical data indicate that water in Budd Lake, the most dilute lake, is predominantly sourced from precipitation. The water in the other three lakes is calcium-magnesium bicarbonate type, reflecting a source of water that has interacted with the landscape. &delta;¹⁸O_H2O and &delta;²H_H2O measurements indicate the lakes are supplied by precipitation modified by evaporation. The water residence time in meromictic Deming Lake is short (100 days), yet it maintains a large reservoir of dissolved iron. Josephine, Arco, and Deming lakes sit in a valley with likely permeable sediments and may be hydrologically connected through wetlands, and recharged with shallow groundwater, as no streams are present. All four lakes develop subsurface chlorophyll maxima layers during the summer. All lakes also develop subsurface oxygen maxima that may result from oxygen trapping in the spring by rapidly developed thermoclines. Documenting the mixing status and general chemistry of these lakes enhances their utility and accessibility for future biogeochemical studies.</p>